PXD011942-2

PXD011942 is an original dataset announced via ProteomeXchange.

Title	The relative intensity of RhPIP2;1 at Ser 273. Membrane proteins were extracted from mock (left) and dehydrated (right) leaves and were used for LC-MS analysis - Part 1
Description	Plants have evolved sophisticated systems to respond to environmental changes, and growth arrest is a common strategy to enhance stress tolerance. Despite the growth-survival tradeoffs is essential for shaping plant productivity, the mechanisms balancing growth and survival remain largely unknown. Aquaporins play a crucial role in growth and stress responses by controlling water transport across membranes. Here, we present that RhPIP2;1, an aquaporin from rose (Rosa sp.), interacts with a membrane-tethered MYB protein, RhPTM. Water deficiency triggers nuclear translocation of RhPTM C-terminus. Silencing of RhPTM causes continuous growth under drought stress, and a consequent decrease in survival rate. RNA-seq indicates that RhPTM influences expression of genes related to carbohydrate metabolism. Water deficiency induces phosphorylation of RhPIP2;1 at Ser273, which is sufficient to promote nuclear translocation of RhPTM C-terminus. These results indicate that RhPIP2;1-RhPTM module serves as a critical player to orchestrate the tradeoff between growth and stress survival in rose.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:51:02.251.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	shuai zhang
SpeciesList	scientific name: Rosa hybrid cultivar; NCBI TaxID: 128735;
ModificationList	dehydrated residue; phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-05 08:15:48	ID requested
1	2019-01-16 00:13:49	announced
⏵ 2	2024-10-22 04:51:02	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Rose

LC-MS

phosphorylation

dehydration

membrane protein

Junping Gao
contact affiliation	Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, China Agricultural University, Beijing, China
contact email	gaojp@cau.edu.cn
lab head
shuai zhang
contact affiliation	China Agricultural University
contact email	s11010256@cau.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011942
     1. Label: PRIDE project
     2. Name: The relative intensity of RhPIP2;1 at Ser 273. Membrane proteins were extracted from mock (left) and dehydrated (right) leaves and were used for LC-MS analysis - Part 1