PXD011941-4

PXD011941 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	50_100uM_DSBU_XL-MS_PHB2(V1)-Myc_HEK293
Description	Mitochondria were isolated from HEK293 cells that stably express PHB2(V1) with an C-terminal 3×Myc tag, and were resuspended in a cross-linking buffer [1 × PBS (pH 7.2) and 50 or 100 μM disuccinimidyl didutyric urea (DSBU) for 30 min at room temperature. The cross-linked mitochondria were then lysed in RIPA buffer, and the clarified supernatants were incubated with anti c-Myc agarose beads. Proteins on the beads were digested by adding trypsin/Lys-C mix for 16 h at 37°C. The resultant peptides were divided into two halves and subjected to LC-MS/MS analysis using both Q Exactive Plus and Orbitrap Fusion Lumos spectrometers. Raw data were analyzed using Proteome Discoverer 2.2 with the XlinkX node to identify cross-linked peptides.
HostingRepository	jPOST
AnnounceDate	2019-09-05
AnnouncementXML	Submission_2022-09-18_03:59:13.379.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hidetaka Kosako
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	unknown modification
Instrument	Q Exactive Plus; Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-05 06:17:42	ID requested
1	2019-09-05 02:54:07	announced
2	2019-09-26 00:03:09	announced	2019-09-26: Updated PubMed.
3	2021-02-04 17:30:52	announced	2021-02-04: Updated FTP location.
⏵ 4	2022-09-18 03:59:14	announced	2022-09-18: Updated FTP location.

Publication List

Yoshinaka T, Kosako H, Yoshizumi T, Furukawa R, Hirano Y, Kuge O, Tamada T, Koshiba T, Structural Basis of Mitochondrial Scaffolds by Prohibitin Complexes: Insight into a Role of the Coiled-Coil Region. iScience, 19():1065-1078(2019) [pubmed]

Yoshinaka T, Kosako H, Yoshizumi T, Furukawa R, Hirano Y, Kuge O, Tamada T, Koshiba T, Structural Basis of Mitochondrial Scaffolds by Prohibitin Complexes: Insight into a Role of the Coiled-Coil Region. iScience, 19():1065-1078(2019) [pubmed]

Keyword List

submitter keyword: cross-linking mass spectrometry

submitter keyword: cross-linking mass spectrometry

Contact List

Hidetaka Kosako
lab head
Hidetaka Kosako
contact affiliation	Tokushima University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000528/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000528/