PXD011923-2

PXD011923 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomics of SPPL2c overexpressing HEK cells
Description	Signal peptide peptidase-like 2c (SPPL2c) is the only member of the GxGD type intramembrane-cleaving aspartyl proteases that so far has not been assigned any substrates and thus its capability of proteolysis and its physiological function remain enigmatic. Based on a surprisingly high expression of SPPL2c in elongated spermatids we applied proteomics on a cellular model system with ectopic expression of SPPL2c and identified a variety of candidate substrates. The majority of these candidate substrates clusters to the biological process of vesicular trafficking. Analysis of selected SNARE proteins reveals proteolytic processing by SPPL2c that impairs vesicular transport and causes retention of cargo proteins in the endoplasmic reticulum. As a consequence, the integrity of subcellular compartments, in particular the Golgi, is disturbed. This may be the trigger for the compartmental reorganization observed during spermatogenesis, which finally results in loss of the majority of endoplasmic reticulum, Golgi and parts of the cytosol in the mature sperm.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:51:02.164.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Mueller
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-03 06:20:16	ID requested
1	2019-02-15 03:59:10	announced
⏵ 2	2024-10-22 04:51:03	announced	2024-10-22: Updated project metadata.

Papadopoulou AA, M, ü, ller SA, Mentrup T, Shmueli MD, Niemeyer J, Haug-Kr, ö, per M, von Blume J, Mayerhofer A, Feederle R, Schr, ö, der B, Lichtenthaler SF, Fluhrer R, Signal peptide peptidase-like 2c impairs vesicular transport and cleaves SNARE proteins. EMBO Rep, 20(3):(2019) [pubmed]

10.15252/embr.201846451;

curator keyword: Biological

submitter keyword: SPPL2c,HEK cells

Regina Fluhrer
contact affiliation	Biomedical Center Institute for Metabolic Biochemistry Ludwig-Maximilians-University Feodor-Lynen Strasse 17 81377 Munich Germany & German Center for Neurodegenerative Diseases (DZNE) Feodor-Lynen Strasse 17 81377 Munich Germany
contact email	regina.fluhrer@med.uni-muenchen.de
lab head
Stephan Mueller
contact affiliation	DZNE Munich Neuroproteomics
contact email	stephan.mueller@dzne.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD011923

PRIDE project URI

• PRIDE
  1. PXD011923
     1. Label: PRIDE project
     2. Name: Quantitative Proteomics of SPPL2c overexpressing HEK cells