PXD011918-3

PXD011918 is an original dataset announced via ProteomeXchange.

Title	WNT5A is transported via lipoprotein particles in the cerebrospinal fluid to regulate hindbrain morphogenesis
Description	WNTs are hydrophobic, lipid-modified proteins that control multiple functions in development and disease via short- and long-range signaling. However, it is unclear how these hydrophobic molecules reach over long distances in the mammalian brain. Here we show that WNT5A is produced by the choroid plexus (ChP) in the developing hindbrain, but not the telencephalon, in both mouse and human. Since the ChP produces and secretes the cerebrospinal fluid (CSF), we examined the presence of WNT5A in the CSF and found it is associated to lipoprotein particles rather than exosomes. Moreover, since the CSF flows along the surface of apical progenitors that do not express Wnt5a, we examined whether WNT5A up- or down-regulates their function. We found that Wnt5a down-regulates proliferation of such progenitor cells. Our study thus identifies the CSF as a route and lipoprotein particles as a vehicle for long-range transport of biologically active WNTs in the central nervous system.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:50:58.797.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Potesil
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-03 02:06:22	ID requested
1	2019-01-14 01:19:07	announced
2	2019-05-27 01:41:44	announced	Updated publication reference for PubMed record(s): 30940800.
⏵ 3	2024-10-22 04:51:06	announced	2024-10-22: Updated project metadata.

10.1038/s41467-019-09298-4;

Kaiser K, Gyllborg D, Proch, á, zka J, Sala, š, ov, á A, Kompan, í, kov, á P, Molina FL, Laguna-Goya R, Radaszkiewicz T, Harno, š J, Proch, á, zkov, á M, Pot, ě, š, il D, Barker RA, Casado Á G, Zdr, á, hal Z, Sedl, á, č, ek R, Arenas E, Villaescusa JC, Bryja V, WNT5A is transported via lipoprotein particles in the cerebrospinal fluid to regulate hindbrain morphogenesis. Nat Commun, 10(1):1498(2019) [pubmed]

curator keyword: Biological

submitter keyword: co-immunoprecipitation,WNT5A, LC-MS/MS

Zbyněk Zdráhal
contact affiliation	CEITEC-MU, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
David Potesil
contact affiliation	Proteomics Core Facility and Research Group Proteomics CEITEC-MU Masaryk University Brno, Czech Republic
contact email	david.potesil@ceitec.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD011918

PRIDE project URI

• PRIDE
  1. PXD011918
     1. Label: PRIDE project
     2. Name: WNT5A is transported via lipoprotein particles in the cerebrospinal fluid to regulate hindbrain morphogenesis