PXD011854 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A specific lipid metabolic profile is associated with the epithelial mesenchymal transition program |
Description | Several studies have identified a specific metabolic program that is associated with the process of epithelial-mesenchymal transition (EMT). Whereas much is known about the association between glucose metabolism and EMT, the contribution of lipid metabolism is not still completely understood. Here, we studied epithelial and mesenchymal breast cancer cells by proteomic and lipidomic approaches and identified significant differences that characterised these models concerning specific metabolic enzymes and metabolites including fatty acids and phospholipids. Higher levels of monounsaturated fatty acids together with increased expression of enzymes of de novo fatty acid synthesis is the distinct signature of epithelial with respect to mesenchymal cells that, on the contrary, show reduced lipogenesis, higher polyunsaturated fatty acids level and increased expression of genes involved in the triglyceride (TAG) synthesis and lipid droplets formation. In the mesenchymal model, the diacylglycerol acyltransferase (DGAT)-1 appears to be the major enzyme involved in TAG synthesis and inhibition of DGAT1, but not DGAT2, drastically reduces the incorporation of labeled palmitate into TAG. Moreover, knockdown of β-catenin demonstrated that this metabolic phenotype in under the control of a network of transcriptional factors and that β-catenin has a specific role in the regulation of lipid metabolism in mesenchymal cells. |
HostingRepository | PRIDE |
AnnounceDate | 2019-01-02 |
AnnouncementXML | Submission_2019-01-02_02:06:00.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Julien Franck |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-11-27 08:23:44 | ID requested | |
⏵ 1 | 2019-01-02 02:06:02 | announced | |
Publication List
Giudetti AM, De Domenico S, Ragusa A, Lunetti P, Gaballo A, Franck J, Simeone P, Nicolardi G, De Nuccio F, Santino A, Capobianco L, Lanuti P, Fournier I, Salzet M, Maffia M, Vergara D, A specific lipid metabolic profile is associated with the epithelial mesenchymal transition program. Biochim Biophys Acta Mol Cell Biol Lipids, 1864(3):344-357(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Epithelial Mesenchymal Transition, Metabolism, Mass spectrometry, Lipids, β-catenin |
Contact List
daniele vergara |
contact affiliation | University of Salento |
contact email | daniele.vergara@unisalento.it |
lab head | |
Julien Franck |
contact affiliation | PRISM-INSERM U1192 |
contact email | julien.franck@univ-lille.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011854
- Label: PRIDE project
- Name: A specific lipid metabolic profile is associated with the epithelial mesenchymal transition program