PXD011848-1

PXD011848 is an original dataset announced via ProteomeXchange.

Title	The bacterial porin OmpA is an inhibitor of Bak-activation at mitochondria and blocks apoptosis of human cells infected with Chlamydia trachomatis
Description	Many infectious agents have the need to inhibit host apoptosis, and various strategies are known. Chlamydia trachomatis is an obligate intracellular bacterium replicating in a vacuole in the human cytosol. Chlamydia-infected human cells are strongly protected against apoptosis. We here mapped this anti-apoptotic activity to the direct blockade of the effectors of mitochondrial apoptosis, the Bcl-2-family proteins Bax and Bak. Molecular analysis of Bak revealed the inhibition of activation-associated conformational changes by the infection. Surprisingly, we identified the C. trachomatis outer membrane porin OmpA in a complex with Bak on mitochondria. When expressed in uninfected human cells, OmpA was imported into mitochondrial membranes. OmpA alone blocked apoptosis and reproduced the changes to Bak-activation observed during infection, reminiscent of the established activity of the human mitochondrial porin, VDAC2. The results suggest that C. trachomatis utilizes the relationship between gram-negative bacteria and mitochondria to block apoptosis and to secure its intracellular growth.
HostingRepository	PRIDE
AnnounceDate	2026-01-26
AnnouncementXML	Submission_2026-01-26_11:36:08.618.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oliver Schilling
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-27 06:53:10	ID requested
⏵ 1	2026-01-26 11:36:09	announced

Dataset with its publication pending

curator keyword: Biological, Biomedical

submitter keyword: OmpA, mitochondria,Chlamydia trachomatis, apoptosis, Bcl-2

Prof. Dr. Oliver Schilling
contact affiliation	Prof. Dr. Oliver Schilling Heisenberg Professor of Translational Proteomics Institute of Surgical Pathology Faculty of Medicine University of Freiburg Breisacher Strasse 115a D-79106 Freiburg, Germany +49 761 27080610 oliver.schilling@mol-med.uni-freiburg.de
contact email	oliver.schilling@mol-med.uni-freiburg.de
lab head
Oliver Schilling
contact affiliation	University of Freiburg
contact email	oliver.schilling@mol-med.uni-freiburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD011848

PRIDE project URI

• PRIDE
  1. PXD011848
     1. Label: PRIDE project
     2. Name: The bacterial porin OmpA is an inhibitor of Bak-activation at mitochondria and blocks apoptosis of human cells infected with Chlamydia trachomatis