PXD011808 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | BAP1 complex promotes transcription by opposing PRC1-mediated H2A ubiquitylation |
Description | In Drosophila, a complex consisting of Calypso and ASX catalyzes H2A deubiquitination and has been reported to act as part of the Polycomb machinery in transcriptional silencing. The mammalian homologs of these proteins (BAP1 and ASXL1/2/3, respectively), are frequently mutated in various cancer types, yet their precise functions remain unclear. Using an integrative approach based on isogenic cell lines generated with CRISPR/Cas9, we uncover an unanticipated role for BAP1 in gene activation. This function requires the assembly of an enzymatically active BAP1-associated core complex (BAP1.com) containing one of the redundant ASXL proteins. We investigate the mechanism underlying BAP1.com-mediated transcriptional regulation and show that it does not participate in Polycomb-mediated silencing. Instead, our results establish that the function of BAP1.com is to safeguard transcriptionally active genes against silencing by the Polycomb Repressive Complex 1. |
HostingRepository | PRIDE |
AnnounceDate | 2019-01-28 |
AnnouncementXML | Submission_2019-01-28_03:57:41.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Guillaume Arras |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-11-23 01:51:41 | ID requested | |
⏵ 1 | 2019-01-28 03:57:43 | announced | |
2 | 2019-01-28 06:33:47 | announced | Updated project metadata. |
3 | 2019-03-06 02:12:13 | announced | Updated project metadata. |
Publication List
Campagne A, Lee MK, Zielinski D, Michaud A, Le Corre S, Dingli F, Chen H, Shahidian LZ, Vassilev I, Servant N, Loew D, Pasmant E, Postel-Vinay S, Wassef M, Margueron R, BAP1 complex promotes transcription by opposing PRC1-mediated H2A ubiquitylation. Nat Commun, 10(1):348(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: BAP, ASXL, PRC1 |
Contact List
Damarys Loew |
contact affiliation | Laboratoire de Spectrometrie de Masse Proteomique Institut Curie PSL Research University |
contact email | Damarys.Loew@curie.fr |
lab head | |
Guillaume Arras |
contact affiliation | Institut Curie |
contact email | guillaume.arras@curie.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011808
- Label: PRIDE project
- Name: BAP1 complex promotes transcription by opposing PRC1-mediated H2A ubiquitylation