PXD011792 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | In vivo proteome of Pseudomonas aeruginosa in airways of cystic fibrosis patients |
Description | Chronic airway infection with P. aeruginosa (PA) is a hallmark of cystic fibrosis (CF) disease. The mechanisms producing PA persistence in CF therapies remain poorly understood. To gain insight on PA physiology in patient airways and better understand how in vivo bacterial functioning differs from in vitro conditions, we investigated the in vivo proteomes of PA in 35 sputum samples from 11 CF patients. We developed a novel bacterial-enrichment method enabling improved identification of PA proteome with CF sputum samples. The in vivo PA proteomes were compared with the proteomes of ex vivo-grown PA populations from the same patient sample. We detected 1528 PA proteins (encoded by 1458 core genes and 70 accessory genes) that were expressed in CF airways, of which 1178 proteins were commonly identified with the ex vivo-grown PA populations. Label-free quantitation and proteome comparison revealed the in vivo up-regulation of siderophore TonB-dependent receptors, remodeling in central carbon metabolism including glyoxylate cycle and lactate utilization, and alginate overproduction. Knowledge of these in vivo proteome differences or others derived using the presented methodology could lead to future treatment strategies aimed at altering PA physiology in vivo to compromise infectivity or improve antibiotic efficacy. |
HostingRepository | PRIDE |
AnnounceDate | 2019-05-09 |
AnnouncementXML | Submission_2019-05-09_00:15:39.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xia Wu |
SpeciesList | scientific name: Pseudomonas aeruginosa; NCBI TaxID: 287; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ FT; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-11-22 01:14:35 | ID requested | |
⏵ 1 | 2019-05-09 00:15:40 | announced | |
Publication List
Wu X, Siehnel RJ, Garudathri J, Staudinger BJ, Hisert KB, Ozer EA, Hauser AR, Eng JK, Manoil C, Singh PK, Bruce JE, In Vivo Proteome of Pseudomonas aeruginosa in Airways of Cystic Fibrosis Patients. J Proteome Res, 18(6):2601-2612(2019) [pubmed] |
Keyword List
curator keyword: Metaproteomics, Biomedical |
submitter keyword: Pseudomonas aeruginosa, cystic fibrosis, quantitative proteomics, bacterial population proteomes |
Contact List
James E. Bruce |
contact affiliation | University of Washington Department of Genome Sciences |
contact email | jimbruce@uw.edu |
lab head | |
Xia Wu |
contact affiliation | University of Washington |
contact email | xiawu2@uw.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011792
- Label: PRIDE project
- Name: In vivo proteome of Pseudomonas aeruginosa in airways of cystic fibrosis patients