PXD011758 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Arhgef11 high and low salt kidney proteomic analysis |
Description | Arhgef11 is a Rho guanine nucleotide exchange factor previously implicated in kidney injury in the Dahl salt-sensitive rat (SS-WT). Through exchange of GDP for GTP, Arhgef11 regulates cytoskeletal structure, function, and cell-cell contacts through a variety of stimuli (e.g., G-protein coupled receptors, growth factors, and shear stress). We studied the SS-Arhgef11-/- rat model at 4-12 weeks of age under low and high salt (0.3% or 2% NaCl). To better understand the molecular mechanisms associated with renal protection from loss of Arhgef11, MS/MS proteomics was performed on kidney from SS-Arhgef11-/- and SS-WT at week 12 (low-salt). At week 12, 1017 genes and 363 proteins were observed. In summary, in vivo phenotyping provides strong evidence that increased Arhgef11 expression in the Dahl S rat leads to actin cytoskeleton mediated changes in cell morphology and cell function (impaired reuptake filtered protein) that promote kidney injury, hypertension, and decline in kidney function. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-08 |
AnnouncementXML | Submission_2021-09-08_07:58:36.150.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Elizabeth Flynn |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-11-20 01:47:56 | ID requested | |
⏵ 1 | 2021-09-08 07:58:37 | announced | |
Publication List
Johnson AC, Wu W, Attipoe EM, Sasser JM, Taylor EB, Showmaker KC, Kyle PB, Lindsey ML, Garrett MR, in the Dahl Salt-Sensitive Rat Protects Against Hypertension-Induced Renal Injury. Hypertension, 75(4):1012-1024(2020) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Arhgef11, high and low salt, kidney function |
Contact List
Elizabeth Flynn |
contact affiliation | University of Mississippi Medical Center 2500 North State Street, G378 Jackson MS 39216-4505 |
contact email | EFlynn@umc.edu |
lab head | |
Elizabeth Flynn |
contact affiliation | University of Mississippi Medical Center |
contact email | EFlynn@umc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD011758 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011758
- Label: PRIDE project
- Name: Arhgef11 high and low salt kidney proteomic analysis