PXD011670-1

PXD011670 is an original dataset announced via ProteomeXchange.

Title	Chromatin capture links the metabolic enzyme AHCY to stem cell proliferation
Description	Profiling the chromatin-bound proteome (chromatome) in a simple, direct, and reliable manner might be key to uncovering the role of yet uncharacterized chromatin factors in physiology and disease. Here, we have design an experimental strategy to survey the chromatome of proliferating cells by using the DNA-mediated chromatin pull-down technology (Dm-ChP). Our approach provides a global view of cellular chromatome in normal physiological conditions and enables the identification of chromatin-bound proteins de novo. Integrating Dm-ChP with genomic and functional data, we have discovered an unexpected chromatin function for adenosylhomocysteinase (AHCY), a major one-carbon pathway metabolic enzyme, in gene activation. Our study reveals a new regulatory axis between the metabolic state of pluripotent cells, ribosomal protein production, and cell division during early phase of embryo development, in which the metabolic flux of methylation reactions is favored in a local milieu
HostingRepository	PRIDE
AnnounceDate	2019-03-13
AnnouncementXML	Submission_2019-03-12_21:33:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eva Borràs
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-12 08:51:18	ID requested
⏵ 1	2019-03-12 21:33:04	announced
2	2024-10-22 04:50:26	announced	2024-10-22: Updated project metadata.

Aranda S, Alcaine-Colet A, Blanco E, Borr, à, s E, Caillot C, Sabid, ó E, Di Croce L, Chromatin capture links the metabolic enzyme AHCY to stem cell proliferation. Sci Adv, 5(3):eaav2448(2019) [pubmed]

curator keyword: Biological

submitter keyword: Chromatin, proteomics, stem cells, AHCY

Eduard Sabidó
contact affiliation	Center for Genomic Regulation (CRG)
contact email	eduard.sabido@crg.eu
lab head
Eva Borràs
contact affiliation	UPF
contact email	eva.borras@upf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD011670

PRIDE project URI

• PRIDE
  1. PXD011670
     1. Label: PRIDE project
     2. Name: Chromatin capture links the metabolic enzyme AHCY to stem cell proliferation