PXD011667-1

PXD011667 is an original dataset announced via ProteomeXchange.

Title	Dirhodium (II) Complex Interferes with Iron-Transport System to Exert Antibacterial Action against Streptococcus pneumoniae
Description	Drug resistance in bacteria is becoming a significant threat to global public health, and the development of novel and efficient antibacterial compounds is urgently needed. Recently, rhodium complexes have attracted attention as antimicrobial agents, yet their antibacterial mechanism remains unknown. In this study, we observed that the dirhodium (II) complex Rh2Ac4 inhibited Streptococcus. pneumoniae growth without significant cytotoxic side-effects on host cells in vitro. We subsequently investigated the antibacterial mechanism of Rh2Ac4 using iTRAQ-based proteomics combined with cellular and biochemical assays. Bioinformatics analysis on the proteomic alterations demonstrated that six molecular functional groups, including metal ion binding and twelve metabolic pathways, were significantly affected after treatment with Rh2Ac4. The interaction network analysis of metal ion binding proteins suggested that Rh2Ac4 decreased the protein expression levels of SPD_1652, SPD_1590 and Gap, which are associated with haem uptake/metabolism. Cellular and biochemical assays further confirmed that Rh2Ac4 could be taken up by bacteria via the PiuABCD haem-uptake system. The structurally similar Rh complex may compete with Fe-haem to decrease Fe-uptake via the PiuABCD system, disrupting iron metabolism to exert its antibacterial activity against S. pneumoniae. These data indicate that Rh2Ac4 is a promising new drug for the treatment of S. pneumoniae infections.
HostingRepository	PRIDE
AnnounceDate	2022-02-28
AnnouncementXML	Submission_2022-02-28_02:32:20.167.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yang Xiao-Yan
SpeciesList	scientific name: Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466); NCBI TaxID: 373153;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-12 08:28:24	ID requested
⏵ 1	2022-02-28 02:32:22	announced
2	2023-11-14 08:49:56	announced	2023-11-14: Updated project metadata.

Yang XY, Xu JY, Meng M, Li N, Liu CY, He QY, Dirhodium (II) complex interferes with iron-transport system to exert antibacterial action against Streptococcus pneumoniae. J Proteomics, 194():160-167(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Dirhodium (II) complex, Streptococcus pneumoniae, iTRAQ, haem-transport system PiuABCD, antibacterial mechanism

Qing-Yu He
contact affiliation	Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan UniversityHealth Engineering, College of Life Science and Technology, Jinan University
contact email	tqyhe@jnu.edu.cn
lab head
Yang Xiao-Yan
contact affiliation	Jinan University
contact email	ouyangxiangyan@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011667
     1. Label: PRIDE project
     2. Name: Dirhodium (II) Complex Interferes with Iron-Transport System to Exert Antibacterial Action against Streptococcus pneumoniae