PXD011639-1

PXD011639 is an original dataset announced via ProteomeXchange.

Title	Host-symbiont proteomes of Bathymodiolus thermophilus
Description	Bathymodiolus mussels inhabiting deep-sea hydrothermal vents harbor bacterial symbionts in their gills, which support the animals’ diet. While the basic mechanisms of energy generation and CO2 fixation that drive these symbioses are largely established, details of molecular interactions between the symbiotic partners and adaptations to their respective habitats remain unknown. In this study, we therefore comparatively examined the genomes and proteomes of two Bathymodiolus hosts and their respective symbionts from different geographical locations. Two mussel species were proteomically compared: i) B. thermophilus mussel containing sulfur-oxidizing symbiont from the east pacific rise. thermophilus and ii) B. azoricus containing thiotrophic and methanotrophic symbionts from the mid-atlantic ridge. Symbionts (for both species) and host components (for B. azoricus) were selectively enriched using a multi-step centrifugation procedure. Enriched host and symbiont fractions along with unenriched gill foot tissue were subject to in-depth semi-quantitative proteomic analyses using the orbitrap and velos mass spectrometers. Proteins were quantified based on their spectral counts using the normalized spectral abundance factor (NSAF) method. We identified common strategies of metabolic interactions that provide mutual nutritional support between host and symbionts, such as the detoxification of ambient sulfide by the Bathymodiolus host, which provides a stable thiosulfate reservoir for the thiotrophic symbionts, and a putative amino acid cycling mechanism that could supply the host with symbiont-derived amino acids. A suite of genes and proteins putatively related to virulence or defense functions was particularly abundant in the B. thermophilus symbiont, compared to its symbiont relatives, and may pose a host species-specific adaptation. Our results reveal both, a high degree of integration between the symbiotic partners, and great potential to adapt to the prevailing environment, which facilitate the holobiont’s survival in its hydrothermal vent habitat.
HostingRepository	PRIDE
AnnounceDate	2019-11-08
AnnouncementXML	Submission_2019-11-08_08:43:33.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011639
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ruby Ponnudurai
SpeciesList	scientific name: Bathymodiolus thermophilus; NCBI TaxID: 12966; scientific name: Bathymodiolus azoricus (Mussel); NCBI TaxID: 150808;
ModificationList	Oxidation
Instrument	LTQ Orbitrap Velos; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-08 23:47:58	ID requested
⏵ 1	2019-11-08 08:43:36	announced

Ponnudurai R, Heiden SE, Sayavedra L, Hinzke T, Kleiner M, Hentschker C, Felbeck H, Sievert SM, Schl, ü, ter R, Becher D, Schweder T, Markert S, Comparative proteomics of related symbiotic mussel species reveals high variability of host-symbiont interactions. ISME J, 14(2):649-656(2020) [pubmed]

Sayavedra L, Kleiner M, Ponnudurai R, Wetzel S, Pelletier E, Barbe V, Satoh N, Shoguchi E, Fink D, Breusing C, Reusch TB, Rosenstiel P, Schilhabel MB, Becher D, Schweder T, Markert S, Dubilier N, Petersen JM, Abundant toxin-related genes in the genomes of beneficial symbionts from deep-sea hydrothermal vent mussels. Elife, 4():e07966(2015) [pubmed]

Ponnudurai R, Kleiner M, Sayavedra L, Petersen JM, Moche M, Otto A, Becher D, Takeuchi T, Satoh N, Dubilier N, Schweder T, Markert S, Metabolic and physiological interdependencies in the Bathymodiolus azoricus symbiosis. ISME J, 11(2):463-477(2017) [pubmed]

curator keyword: Biological

submitter keyword: Bathymodiolus, symbiosis, symbiont, proteome, host, hydrothermal vent

Thomas Schweder, Stephanie Markert
contact affiliation	University of Greifswald, Institut für Pharmazie/C_DAT, Pharmazeutische Biotechnologie, Felix-Hausdorff-Str. 3, 17489 Greifswald, Germany
contact email	thomas.schweder@uni-greifswald.de
lab head
Ruby Ponnudurai
contact affiliation	University of Greifswald
contact email	rp114410@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD011639

PRIDE project URI

• PRIDE
  1. PXD011639
     1. Label: PRIDE project
     2. Name: Host-symbiont proteomes of Bathymodiolus thermophilus