PXD011622 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Typing complex meningococcal vaccines to understand diversity and population structure of key vaccine antigens |
Description | Vaccines to prevent capsular group B meningococcal disease have been based on proteins, as the polysaccharide capsule was deemed unsuitable due to its similarity to human tissues. Outer membrane vesicle (OMV) vaccines have been targeted at specific capsular group B epidemics in Cuba, Norway, and New Zealand, with the major immunogen determined as porin A (PorA). Subcapsular protein vaccines aim to increase breadth of coverage of meningococcal strains, by the inclusion of protein variants from many different clonal complexes (ccs). Using scalable and portable genomic techniques, it is possible to study the diversity of OMV and subcapsular proteins in relevant meningococcal populations. Shotgun proteomics identified 461 proteins in the OMVs derived from NZ98/254, a component of the Bexsero® vaccine, with a complex proteome comprised of outer membrane proteins and proteins from other cellular compartments. Amino acid sequences for 24 selected proteins were catalogued within the PubMLST Neisseria database as part of the OMV peptide Typing (OMVT) scheme. Of the 24 proteins included, there was variation in the extent of diversity and association with ccs from the most conserved peptides FbpA (NEISp0578) and putative periplasmic proteins (NEISp1063) to the most diverse TbpA (NEISp1690). There were 1752 OMVTs identified amongst 2492/3506 isolates. OMVTs were further grouped into clusters (identical at ≥18 peptide sequences), 45.3% of isolates were assigned to one of 27 OMVT clusters. Both OMVTs and clusters were strongly associated with cc, genogroup and recombinant Bexsero® antigens. The OMVT scheme represents an open-access, web-based tool for the systematic analysis of the multiple components of OMV vaccines, demonstrating that combinations of OMV proteins exist in discrete, non-overlapping combinations associated with both genogroup and Bexsero® Antigen Sequence type (BAST). This highly structured population of disease-causing meningococci is consistent with proposed effects of host immune selection and competition between allelic variants on meningococcal population structure. This has implications for future vaccine assessment, and development, especially the choice of antigen combinations. The methodology is portable and will facilitate region-specific WGS interrogation, allowing informed choices about vaccine development or implementation. |
HostingRepository | PRIDE |
AnnounceDate | 2018-11-14 |
AnnouncementXML | Submission_2019-05-27_02:08:08.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD011622 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Jun Wheeler |
SpeciesList | scientific name: Neisseria meningitidis serogroup B; NCBI TaxID: 491; |
ModificationList | TMT6plex; Oxidation; Carbamidomethyl |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-11-08 03:30:30 | ID requested | |
1 | 2018-11-14 03:42:21 | announced | |
⏵ 2 | 2019-05-27 02:08:09 | announced | Updated publication reference for PubMed record(s): 30687793. |
Publication List
Rodrigues CMC, Chan H, Vipond C, Jolley K, Harrison OB, Wheeler J, Whiting G, Feavers IM, Maiden MCJ, Typing complex meningococcal vaccines to understand diversity and population structure of key vaccine antigens. Wellcome Open Res, 3():151(2018) [pubmed] |
Keyword List
submitter keyword: Outer membrane vesicle, Neisseria meningitidis, meningococcal, vaccines, shotgun proteomics, LC-MS/MS |
Contact List
Jun Wheeler |
contact affiliation | National Institute for Biological Standards and Control |
contact email | jun.wheeler@nibsc.org |
lab head | |
Jun Wheeler |
contact affiliation | NIBSC |
contact email | jun.wheeler@nibsc.org |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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- PRIDE
- PXD011622
- Label: PRIDE project
- Name: Typing complex meningococcal vaccines to understand diversity and population structure of key vaccine antigens