PXD011607-1

PXD011607 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Protein O-glycoproteome in Streptomyces coelicolor
Description	The membrane O-glycoproteome in Streptomyces coelicolor J1929 was isolated and characterised by LC-ESI-MS/MS, using CID, HCD and ETD fragmentation. Streptomyces coelicolor J1929 was grown in phosphate limiting (F134) medium, and the membrane proteins were isolated after 20, 35, 43 and 60 h of growth. Glycoproteins were enriched using lectin affinity chromatography (Con A) and the enriched fractions were analysed by LC-ESI-CID-MS/MS on a Bruker maXis mass spectromter. The enriched glycoproteins isolated after 43 h of growth were further analysed by LC-MS/MS on an Orbitrap Fusion Tribrid mass spectrometer. The S. coelicolor glycopeptides in this experiment were analysed using both HCD and ETD fragmentation, and using both the ion trap (low resolution, high speed - mass accuracy < 0.5 Da) and Orbitrap (high resolution, lower speed - mass accuracy < 3 ppm) mass analysers. Four different data acquisitions were performed to include these options: MS in Orbitrap, HCD measured in ion trap (HCD_IT), MS in Orbitrap, ETD measured ion trap (ETD_IT), MS in Orbitrap, ETD measured in Orbitrap (ETD_OT), MS in Orbitrap, HCD measured in ion trap and ETD measured in Orbitrap (HCD_IT; ETD_OT).
HostingRepository	MassIVE
AnnounceDate	2019-06-25
AnnouncementXML	Submission_2019-06-25_06:00:36.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tessa Keenan
SpeciesList	scientific name: Streptomyces coelicolor A3(2) J1929;
ModificationList	O-mannosyl-L-serine; O-mannosyl-L-threonine
Instrument	maXis; Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-07 06:25:16	ID requested
⏵ 1	2019-06-25 06:00:37	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Streptomyces coelicolor, O-glycoproteome, membrane glycoproteome, protein O-mannosylation

submitter keyword: Streptomyces coelicolor, O-glycoproteome, membrane glycoproteome, protein O-mannosylation

Contact List

Professor Maggie Smtih
contact affiliation	University of York
contact email	maggie.smith@york.ac.uk
lab head
Tessa Keenan
contact affiliation	University of York
contact email	tessa.keenan@york.ac.uk
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000083115