PXD011604-1

PXD011604 is an original dataset announced via ProteomeXchange.

Title	Proteomic Characterization of Bradyrhizobium diazoefficiens bacteroids during soybean root nodules senescence
Description	The form and physiology of Bradyrhizobium diazoefficiens after the decline of symbiotic nitrogen fixation has been characterized. Proteomic analyses showed that the post-symbiotic B. diazoefficiens undergo metabolic remodeling as well defined groups of proteins declined, increased or remained unchanged from 56 to 119 days after planting suggesting a transition to a hemibiotrophic-like lifestyle. Enzymatic analysis showed distinct patterns in both the cytoplasm and the periplasm. Similar to the bacteroid, the post-symbiotic bacteria rely on a non-citric acid cycle supply of succinate and although viable, they did not demonstrate the ability to grow within the senescent nodule.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-12_15:31:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Emerich
SpeciesList	scientific name: Bradyrhizobium japonicum USDA 110; NCBI TaxID: 224911;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-07 06:11:41	ID requested
⏵ 1	2019-11-12 15:31:44	announced
2	2024-10-22 04:50:15	announced	2024-10-22: Updated project metadata.

Strodtman KN, Frank S, Stevenson S, Thelen JJ, Emerich DW, Bacteroids Reveals a Post-Symbiotic, Hemibiotrophic-Like Lifestyle of the Bacteria within Senescing Soybean Nodules. Int J Mol Sci, 19(12):(2018) [pubmed]

curator keyword: Biological

submitter keyword: Bradyrhizobium diazoefficiens, soybean, nitrogen fixation, senescence

David W. Emerich
contact affiliation	Department of Biochemistry, University of Missouri, Columbia, Missouri
contact email	EmerichD@Missouri.edu
lab head
David Emerich
contact affiliation	University of Missouri
contact email	emerichd@missouri.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD011604

PRIDE project URI

• PRIDE
  1. PXD011604
     1. Label: PRIDE project
     2. Name: Proteomic Characterization of Bradyrhizobium diazoefficiens bacteroids during soybean root nodules senescence