PXD011598-3

PXD011598 is an original dataset announced via ProteomeXchange.

Title	QUANTITATIVE PROTEOME PROFILING OF NEUROSPORA CRASSA REVEALS A CELLOBIOSE-DEPENDENT NETWORK COORDINATING LIGNOCELLULOLYTIC RESPONSES
Description	Cellulose, particularly the major cellulolytic product cellobiose, can induce the production of enzymes associated with deconstruction of lignocellulose in filamentous fungi. However, the detailed mechanisms underlying this biotechnologically important process remain to be disclosed. Here, the proteome response to cellobiose, crystalline cellulose (Avicel), and carbon starvation of a Neurospora crassa triple β-glucosidase mutant were compared using tandem mass tag (TMT)-based proteome quantification. Improved quantification accuracy was achieved with synchronous precursor selection (SPS)-based MS3 technology compared to MS2 using a high resolution tribrid mass spectrometer. Exposure to carbon starvation, cellobiose or Avicel induced the production of cellulase and lytic polysaccharide monooxygenase enzymes in N. crassa, as well as a cellobionic acid transporter, indicating their functional roles in the early adaptation to plant cell wall. In particular, cellobiose specifically induced the production of proteins in the functional categories of protein processing and export as well as cell wall organization. The data presented here integrates the signaling pathway associated with cellobiose transporters CDT-1 and/or CDT-2 with the direct targets of the transcription factors CLR-1, CLR-2, and XLR-1, the unfolded protein response (UPR) mediated by Ire-1/Hac-1, as well as calcium homeostasis and cell wall organization. The cellobiose-dependent response network will be useful for rational strain improvement to facilitate the production of lignocellulases in filamentous fungi and plant biomass-based products.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:03:46.522.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shaolin Chen
SpeciesList	scientific name: Neurospora crassa OR74A; NCBI TaxID: 367110;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-07 05:16:52	ID requested
1	2020-05-15 00:52:05	announced
2	2020-08-11 03:19:25	announced	2020-08-11: Updated publication reference for PubMed record(s): 32471912.
⏵ 3	2024-10-22 05:03:51	announced	2024-10-22: Updated project metadata.

10.1128/aem.00653-20;

Liu D, Liu Y, Zhang D, Chen X, Liu Q, Xiong B, Zhang L, Wei L, Wang Y, Fang H, Liesche J, Wei Y, Glass NL, Hao Z, Chen S, Quantitative Proteome Profiling Reveals Cellobiose-Dependent Protein Processing and Export Pathways for the Lignocellulolytic Response in Neurospora crassa. Appl Environ Microbiol, 86(15):(2020) [pubmed]

curator keyword: Biological

submitter keyword: Quantitative proteomics, Cellulase secretion, SPS-MS3,Cellobiose, Cellulose, UPR, Protein Trafficking, TMT-labeling, Cellulase expression

Shaolin Chen
contact affiliation	Northwest A&F University, Yangling, Shaanxi, China
contact email	slc1916@nwsuaf.edu.cn
lab head
Shaolin Chen
contact affiliation	Northwest A&F University
contact email	slc1916@nwsuaf.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD011598

PRIDE project URI

• PRIDE
  1. PXD011598
     1. Label: PRIDE project
     2. Name: QUANTITATIVE PROTEOME PROFILING OF NEUROSPORA CRASSA REVEALS A CELLOBIOSE-DEPENDENT NETWORK COORDINATING LIGNOCELLULOLYTIC RESPONSES