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PXD011591-2

PXD011591 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe ABCB7-like Transporter PexA in Rhodobacter capsulatus maintains proper Distribution of Reactive Sulfur Species
DescriptionThe mitochondrial ATP-binding cassette (ABC) transporters ABCB7 in humans, Atm1 in yeast and ATM3 in plants are highly conserved in their overall architecture and particularly in their glutathione binding pocket located within the transmembrane spanning domains. These transporters have attracted interest in the last two decades based on their proposed role in connecting the mitochondrial iron-sulfur (Fe-S) cluster assembly with its cytosolic Fe-S cluster assembly (CIA) counterpart. So far, the specific compound that is transported across the membrane remains unknown. In this report we characterized the ABCB7-like transporter Rcc02305 in Rhodobacter capsulatus, which shares 47% amino acid sequence identity to its mitochondrial counterpart. The constructed interposon mutant strain in R. capsulatus displayed increased levels of intracellular reactive oxygen species without a simultaneous accumulation of the cellular iron concentration. The inhibition of endogenous glutathione biosynthesis resulted in an increase of total glutathione levels in the mutant strain. Bioinformatic analysis of the amino acid sequence motifs revealed an aminotransferase class-V pyridoxal-5’-phosphate (PLP) binding site overlapping with the Walker A motif within the nucleotide binding domains of the transporter. The PLP cofactor is well characterized in L-cysteine desulfurases like IscS and NFS1 for its role in the formation of a protein-bound persulfide group. In this report, we present a model proposing that this ABC-transporter in R. capsulatus in its inward facing open conformation first produces a nucleophilic persulfide, which further reacts in a substitution reaction with a trapped GS(S)nG bound to the TMD resulting to a mixed glutathione polysulfide. In the next step, the release of the persulfide from the PLP binding site enables ATP to bind to the Walker A motif and the subsequent hydrolysis of ATP triggers a translocation of the mixed disulfide to the periplasm. We rename the ABCB7-like transporter Rcc02305 in R. capsulatus to PexA for PLP binding exporter.
HostingRepositoryPRIDE
AnnounceDate2019-03-08
AnnouncementXMLSubmission_2019-04-03_07:24:46.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBeata Siemiatkowska
SpeciesList scientific name: Rhodobacter capsulatus; NCBI TaxID: 1061;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-11-06 04:59:50ID requested
12019-03-08 06:12:49announced
22019-04-03 07:24:48announcedUpdated publication reference for PubMed record(s): 30918498.
32024-10-22 04:50:44announced2024-10-22: Updated project metadata.
Publication List
Riedel S, Siemiatkowska B, Watanabe M, M, ü, ller CS, Sch, ü, nemann V, Hoefgen R, Leimk, ü, hler S, Is Involved in the Translocation of Reactive Sulfur Species. Front Microbiol, 10():406(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: reactive sulfur species, ABCB7, persulfide, polysulfide, glutathione, ABC transporter, reactive oxygen species, Walker A motif, pyridoxal-5’-phosphate
Contact List
Silke Leimkühler
contact affiliationInstitute of Biochemistry and Biology, Department of Molecular Enzymology, University of Potsdam, Potsdam, Germany
contact emailsleim@uni-potsdam.de
lab head
Beata Siemiatkowska
contact affiliationMax Planck Institute of Molecular Plant Physiology
contact emailSiemiatkowska@mpimp-golm.mpg.de
dataset submitter
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