PXD011556-1

PXD011556 is an original dataset announced via ProteomeXchange.

Title	Bacteriocin LC-MSMS .
Description	This is the first report of a bacteriocin being produced by lactobacillus acidophilus ATCC 4356. For protein identification, the ~8-kDa peptide band was removed from the acrylamide gel and digested in-gel by trypsin, and the resulting peptide fragments were extracted and analyzed by LC-MS/MS analysis. The MS data were processed using Thermo Proteome Discoverer software (v2.2) with the SEQUEST search engine. Three peptides, VAHCASQIGR (amino acids 23-32), GSAACVSYLTR (amino acids 69-79), GSAACVSYLTRHRHH (amino acids 69-83) were identified as tryptic fragments on the basis of LC-MS/MS.This is the first report of a bacteriocin being produced by lactobacillus acidophilus ATCC 4356. For protein identification, the ~8-kDa peptide band was removed from the acrylamide gel and digested in-gel by trypsin, and the resulting peptide fragments were extracted and analyzed by LC-MS/MS analysis. The MS data were processed using Thermo Proteome Discoverer software (v2.2) with the SEQUEST search engine. Three peptides, VAHCASQIGR (amino acids 23-32), GSAACVSYLTR (amino acids 69-79), GSAACVSYLTRHRHH (amino acids 69-83) were identified as tryptic fragments on the basis of LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:20:26.038.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sima Modiri
SpeciesList	scientific name: Lactobacillus acidophilus; NCBI TaxID: 1579;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-01 03:13:06	ID requested
⏵ 1	2024-10-07 10:20:26	announced

10.1128/aem.00367-20;

Modiri S, Kasra Kermanshahi R, Soudi MR, Arab SS, Khammari A, Cousineau B, Vali H, Zahiri HS, Noghabi KA, through Membrane Perturbation and Virulence Attenuation: Experimental Results Confirm Molecular Dynamics Simulation. Appl Environ Microbiol, 86(10):(2020) [pubmed]

curator keyword: Biological

submitter keyword: Lactobacillus acidophilus atcc 4356,Bacteriocin

Kambiz Akbari Noghabi
contact affiliation	Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), P. O. Box 14155-6343, Tehran, Iran
contact email	akbari@nigeb.ac.ir
lab head
Sima Modiri
contact affiliation	NIGEB
contact email	modiri.sima@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD011556

PRIDE project URI

• PRIDE
  1. PXD011556
     1. Label: PRIDE project
     2. Name: Bacteriocin LC-MSMS