PXD011555-2

PXD011555 is an original dataset announced via ProteomeXchange.

Title	A ¨omic¨ approach to Pyrocystis lunula
Description	Pyrocystis lunula (Schutt) is a photoautotrophic unarmoured dinoflagellate, commonly found in marine environments. Today it exist several biotechnological applications derived from the bioluminescent system of this species. From a post-genomic perspective, and in order to study the whole proteome of P. lunula, a ¨omic¨ approach (transcriptomic-proteomic analysis) was initiated using fresh microalgae samples. A total of 80.875.390 reads were generated (400.000 conting, XXX pb) and 17.461 peptides were detected, getting 3.182 protein identification hits. The identified proteins were categorized according to functional description and gene ontology classification. In this study, it has been developed and described the first proteomic analysis of the microalgae P. lunula. In addition to shed light on a series of important factors involved with the processes of regulation of gene expression. The presence of the luciferin-binding protein (LBP), which had not been described so far in Pyrocystis, is highlighted.
HostingRepository	PRIDE
AnnounceDate	2019-10-10
AnnouncementXML	Submission_2019-10-10_08:54:04.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francisco Fernández-Acero
SpeciesList	scientific name: Pyrocystis lunula; NCBI TaxID: 2972;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-01 02:59:49	ID requested
1	2019-04-12 04:45:51	announced
⏵ 2	2019-10-10 08:54:05	announced	2019-10-10: Updated project metadata.

Fajardo C, Amil-Ruiz F, Fuentes-Almagro C, De Donato M, Martinez-Rodriguez G, Escobar-Ni, ñ, o A, Carrasco R, Mancera JM, Fernandez-Acero FJ, An "omic" approach to Pyrocystis lunula: New insights related with this bioluminescent dinoflagellate. J Proteomics, 209():103502(2019) [pubmed]

submitter keyword: Pyrocystis lunula, transcriptomics, proteomics, LBP, bioluminiscence

Francisco Javier Fernández Acero
contact affiliation	Microbiology department, IVAGRO, UCA
contact email	franciscojavier.fernandez@uca.es
lab head
Francisco Fernández-Acero
contact affiliation	University of Cadiz
contact email	franciscojavier.fernandez@uca.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD011555

PRIDE project URI

• PRIDE
  1. PXD011555
     1. Label: PRIDE project
     2. Name: A ¨omic¨ approach to Pyrocystis lunula