PXD011552 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | SUMO conjugation to the pattern recognition receptor FLS2 triggers intracellular signalling in plant innate immunity |
Description | Arabidopsis lines expressing 35S::Strep-SUMO1H89R in Col-0 background were generated since the H89R SUMO1 variant simplifies MS/MS detection of SUMO conjugated lysines after trypsinization (Miller et al., 2010). Transgenic seedlings were treated with water or 250nM flg22 for 30 minutes and total protein extracts including membrane fractions were affinity purified with Pierceā¢ Streptavidin Magnetic Beads. LC-MS analysis of eluting proteins allowed identification of candidate SUMO-conjugated proteins in response to flg22 treatment. |
HostingRepository | PRIDE |
AnnounceDate | 2019-11-12 |
AnnouncementXML | Submission_2019-11-12_15:22:00.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Adrian Brown |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-11-01 02:58:52 | ID requested | |
⏵ 1 | 2019-11-12 15:22:01 | announced | |
Publication List
Orosa B, Yates G, Verma V, Srivastava AK, Srivastava M, Campanaro A, De Vega D, Fernandes A, Zhang C, Lee J, Bennett MJ, Sadanandom A, SUMO conjugation to the pattern recognition receptor FLS2 triggers intracellular signalling in plant innate immunity. Nat Commun, 9(1):5185(2018) [pubmed] |
Keyword List
submitter keyword: Arabidopsis thaliana, seedlings, SUMO, FLS2, LC-MS/MS, TripleTOF 6600 |
Contact List
Ari Sadanandom |
contact affiliation | Department of Biosciences, Durham University Durham, UK |
contact email | ari.sadanandom@durham.ac.uk |
lab head | |
Adrian Brown |
contact affiliation | Durham University |
contact email | a.p.brown@durham.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD011552 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011552
- Label: PRIDE project
- Name: SUMO conjugation to the pattern recognition receptor FLS2 triggers intracellular signalling in plant innate immunity