PXD011522-1

PXD011522 is an original dataset announced via ProteomeXchange.

Title	Galectin-8 facilitates B cell responses to surface-tethered antigens by promoting antigen extraction at the B cell synapse
Description	Complete activation of B cells relies on their capacity to extract surface-tethered 35  antigens at the immune synapse by either exerting mechanical forces or promoting 36  their proteolytic degradation through lysosome secretion. Whether antigen extraction 37  can be tuned by local cues originating from the lymphoid microenvironment has not 38  been investigated. We here show that Galectin-8 – an extracellular glycan-binding 39  protein that regulates interactions between cells and matrix proteins – facilitates 40  synapse formation upon BCR recognition of surface-tethered antigens. As a 41  consequence of this, B cell arrest phases are longer in the presence of Galectin-8, 42  which translates in increased antigen extraction and presentation to T lymphocytes in 43  vivo. We further show that Galectin-8 triggers a faster recruitment and secretion of 44  lysosomes at the B cell-antigen contact site, suggesting that enhanced antigen 45  extraction in the presence of Galectin-8 might result from increased antigen 46  proteolysis. Thus, extracellular cues can determine how B cells sense and extract 47  surface-tethered antigens and thereby tune B cell responses in vivo.
HostingRepository	PRIDE
AnnounceDate	2018-12-14
AnnouncementXML	Submission_2018-12-14_03:16:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibaut LEGER
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-30 02:48:14	ID requested
⏵ 1	2018-12-14 03:16:44	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: B-lymphocytes, Immune synapse, Galectin-8, Antigen extraction, processing and presentation, Polarity, Mouse immunization

Dr María Isabel Yuseff
contact affiliation	Pontificia Universidad Católica de Chile Department of Cellular and Molecular Biology Av. B. O’Higgins 340. Santiago, Chile Phone: 56-2-23541843
contact email	myuseff@bio.puc.cl
lab head
Thibaut LEGER
contact affiliation	Institut Jacques Monod
contact email	thibaut.leger@ijm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/12/PXD011522

PRIDE project URI

• PRIDE
  1. PXD011522
     1. Label: PRIDE project
     2. Name: Galectin-8 facilitates B cell responses to surface-tethered antigens by promoting antigen extraction at the B cell synapse