PXD011519-1

PXD011519 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry of Phage PhiKZ gp54 with the prohead protease gp175
Description	Assay of recombinant Pseudomonas phage PhiKZ gp54 with the prohead protease gp175. The gene for each protein was cloned into the expression plasmid DH10B. The assay was conducted in a lysate of E. coli DH10B cells, conditions in which PhiKZ gp175 had previously been shown to be active. Analyses of gel slices identified C-terminal processing of gp54 via the semi-tryptic peptide 558-FITAMDAAAAAAGVTVTME-576 in the gp54-plus-gp175 slices (predominantly slice 3) provided convincing evidence for proteolysis of gp54 in the presence of gp175.
HostingRepository	PRIDE
AnnounceDate	2018-11-21
AnnouncementXML	Submission_2018-11-21_14:16:02.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011519
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Julie Thomas
SpeciesList	scientific name: Pseudomonas phage phiKZ; NCBI TaxID: 169683;
ModificationList	Ammonia-loss; Deamidated; Dehydrated; Oxidation; Acetyl; Carbamidomethyl
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-30 02:37:44	ID requested
⏵ 1	2018-11-21 14:16:02	announced
2	2019-05-27 02:09:10	announced	Updated publication reference for PubMed record(s): 30541839.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Pseudomonas phage PhiKZ, prohead protease

Julie Thomas
contact affiliation	Rochester Institute of Technology
contact email	jatsbi@rit.edu
lab head
Julie Thomas
contact affiliation	Rochester Institute of Technology
contact email	jatsbi@rit.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD011519

PRIDE project URI

• PRIDE
  1. PXD011519
     1. Label: PRIDE project
     2. Name: Mass spectrometry of Phage PhiKZ gp54 with the prohead protease gp175