PXD011519 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mass spectrometry of Phage PhiKZ gp54 with the prohead protease gp175 |
Description | Assay of recombinant Pseudomonas phage PhiKZ gp54 with the prohead protease gp175. The gene for each protein was cloned into the expression plasmid DH10B. The assay was conducted in a lysate of E. coli DH10B cells, conditions in which PhiKZ gp175 had previously been shown to be active. Analyses of gel slices identified C-terminal processing of gp54 via the semi-tryptic peptide 558-FITAMDAAAAAAGVTVTME-576 in the gp54-plus-gp175 slices (predominantly slice 3) provided convincing evidence for proteolysis of gp54 in the presence of gp175. |
HostingRepository | PRIDE |
AnnounceDate | 2018-11-21 |
AnnouncementXML | Submission_2018-11-21_14:16:02.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD011519 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Julie Thomas |
SpeciesList | scientific name: Pseudomonas phage phiKZ; NCBI TaxID: 169683; |
ModificationList | Ammonia-loss; Deamidated; Dehydrated; Oxidation; Acetyl; Carbamidomethyl |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-10-30 02:37:44 | ID requested | |
⏵ 1 | 2018-11-21 14:16:02 | announced | |
2 | 2019-05-27 02:09:10 | announced | Updated publication reference for PubMed record(s): 30541839. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Pseudomonas phage PhiKZ, prohead protease |
Contact List
Julie Thomas |
contact affiliation | Rochester Institute of Technology |
contact email | jatsbi@rit.edu |
lab head | |
Julie Thomas |
contact affiliation | Rochester Institute of Technology |
contact email | jatsbi@rit.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011519
- Label: PRIDE project
- Name: Mass spectrometry of Phage PhiKZ gp54 with the prohead protease gp175