PXD011510 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Critical role of the UBL-domain in stimulating the E3 ubiquitin ligase activity of UHRF1 towards chromatin |
Description | The RING E3 ubiquitin ligase UHRF1 controls DNA methylation through its ability to target the maintenance DNA methyltransferase DNMT1 to newly replicated chromatin. DNMT1 recruitment relies on ubiquitylation of histone H3 by UHRF1, however, how UHRF1 deposits ubiquitin onto the histone is unknown. Here, we demonstrate that the ubiquitin-like domain (UBL) of UHRF1 is essential for RINGmediated H3 ubiquitylation. Using chemical crosslinking and mass spectrometry, biochemical assays and recombinant chromatin substrates we show that the UBL participates in structural rearrangements of UHRF1 upon binding to chromatin and the E2 ubiquitin conjugating enzyme UbcH5a/UBE2D1. Similar to ubiquitin, the UBL exerts its effects through a hydrophobic patch that contacts a regulatory surface on the “backside” of the E2 to stabilise the E2-E3-chromatin complex. Our analysis of the enzymatic mechanism of UHRF1 uncovers an unexpected function of the UBLdomain and defines a new role for this domain in DNMT1-dependent inheritance of DNA methylation. |
HostingRepository | PRIDE |
AnnounceDate | 2018-11-04 |
AnnouncementXML | Submission_2018-11-04_15:40:43.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Holger Kramer |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | ubiquitinylated lysine; propanoyl labeling reagent light form (N-term); propanoylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos; LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-10-29 07:11:46 | ID requested | |
⏵ 1 | 2018-11-04 15:40:44 | announced | |
2 | 2018-11-20 01:15:50 | announced | Updated publication reference for PubMed record(s): 30392929. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: UHRF1, chromatin, ubiquitylation, Ubiquitin-like fold, DNA methylation, Histone modification |
Contact List
Till Bartke |
contact affiliation | Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Ingolstädter Landstr. 1 D-85764 Neuherberg Germany |
contact email | till.bartke@helmholtz-muenchen.de |
lab head | |
Holger Kramer |
contact affiliation | MRC London Institute of Medical Sciences |
contact email | h.kramer@lms.mrc.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011510
- Label: PRIDE project
- Name: Critical role of the UBL-domain in stimulating the E3 ubiquitin ligase activity of UHRF1 towards chromatin