PXD011499-1

PXD011499 is an original dataset announced via ProteomeXchange.

Title	A proteomics approach to study the role of lipid droplets in plant defense
Description	Lipid Droplets (LDs), classically viewed as lipid storage particles, are recently emerging as dynamic organelles associated with stress responses and development; however, their involvement in plant immunity has been little investigated. Here we studied LDs in Arabidopsis thaliana leaves that respond to Pseudomonas syringae pv. tomato DC3000 avrRpm1 by inducing a hypersensitive defense reaction (HR), as well as during senescence. We established a protocol to reproducibly isolate LDs from a limited amount of tissue and performed a proteomic analysis of LDs from Pseudomonas-infected (for 24 h and 72 h) and senescent leaves. The proteomes defined shared ~50% similarity, with an enrichment of proteins associated with the Gene Ontology terms “transport” and “responses to stress”. As validation, we confirmed LD localization of glycerol-3-phosphate-acetyltransferase 8 (GPAT8) and GPAT4, enzymes required for e.g., cutin biosynthesis, by transient expression of GFP fusions in HR-induced leaves of Nicotiana benthamiana. Similarly, we proved the localization to LDs of aldehyde dehydrogenase 3F1 (ALDH3F1), α-dioxygenase1 (α-DOX1) and caleosin3 (CLO3), all involved in the synthesis of fatty acid derivatives. Moreover, we found that PAD3 (phytoalexin deficient 3) was distributed in patches along the ER and plasma membrane, and in LDs near dead cells. The nature of these proteins and of the additional components characterized in the LDs, together with the phenotypic examination of selected mutants, established a functional link between LDs and plant immunity. Our data suggest that these organelles participate in protein trafficking and in lipid changes that affect the interaction of plants with invading pathogens.
HostingRepository	PRIDE
AnnounceDate	2022-02-23
AnnouncementXML	Submission_2022-02-23_08:53:49.548.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011499
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alberto Paradela
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	Glu->pyro-Glu; Deamidated; Oxidation; Acetyl; Carbamidomethyl; Gln->pyro-Glu
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-29 04:44:55	ID requested
⏵ 1	2022-02-23 08:53:49	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: lipid droplets/Pseudomonas/HR-induced defense/senescence/Arabidopsis

Carmen Castresana
contact affiliation	Centro Nacional de Biotecnología (CNB-CSIC), Darwin 3, E 28049 Madrid, Spain
contact email	ccastresana@cnb.csic.es
lab head
Alberto Paradela
contact affiliation	CNB-CSIC
contact email	alberto.paradela@cnb.csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD011499

PRIDE project URI

• PRIDE
  1. PXD011499
     1. Label: PRIDE project
     2. Name: A proteomics approach to study the role of lipid droplets in plant defense