PXD011491-1

PXD011491 is an original dataset announced via ProteomeXchange.

Title	In vitro and in vivo proteomic comparison of human neural progenitor cell-induced photoreceptor survival
Description	Retinal degenerative diseases are some of the leading causes of blindness with few treatments. Various cell-based therapies have aimed to slow the progression of vision loss by preserving light-sensing photoreceptor cells. A subretinal injection of human neural progenitor cells (hNPCs) into the Royal College of Surgeons (RCS) rat model of retinal degeneration has aided in photoreceptor survival, though the mechanisms are mainly unknown. Identifying the retinal proteomic changes that occur following hNPC treatment will lead to better understanding of neuroprotection. To mimic the retinal environment following hNPC injection, a co-culture system of retinas and hNPCs was developed. Less cell death occurred in RCS retinal tissue co-cultured with hNPCs than in retinas cultured alone, suggesting that hNPCs provide retinal protection in vitro. Comparison of ex vivo and in vivo retinas identified NRF2-mediated oxidative response signaling as an hNPC-induced pathway. This is the first study to compare proteomic changes following treatment with hNPCs in both an ex vivo and in vivo environment, further allowing the use of ex vivo modeling for mechanisms of retinal preservation. Elucidation of the protein changes in the retina following hNPC treatment may lead to the discovery of mechanisms of photoreceptor survival and its therapeutic for clinical applications.
HostingRepository	PRIDE
AnnounceDate	2018-12-17
AnnouncementXML	Submission_2018-12-17_05:47:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Melissa Jones
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-29 02:51:30	ID requested
⏵ 1	2018-12-17 05:47:41	announced
2	2024-10-22 04:50:03	announced	2024-10-22: Updated project metadata.

Jones MK, Lu B, Chen DZ, Spivia WR, Mercado AT, Ljubimov AV, Svendsen CN, Van Eyk JE, Wang S, In Vitro and In Vivo Proteomic Comparison of Human Neural Progenitor Cell-Induced Photoreceptor Survival. Proteomics, 19(3):e1800213(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Neural progenitors, retina, retinal degeneration, stem cells, cell therapy

Shaomei Wang
contact affiliation	Cedars-Sinai Medical Center
contact email	shaomei.wang@cshs.org
lab head
Melissa Jones
contact affiliation	UCSD
contact email	mkj006@ucsd.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/12/PXD011491

PRIDE project URI

• PRIDE
  1. PXD011491
     1. Label: PRIDE project
     2. Name: In vitro and in vivo proteomic comparison of human neural progenitor cell-induced photoreceptor survival