PXD011476-1
PXD011476 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Extended sialylated O-glycan repertoire of human urinary glycoproteins discovered and characterized using EThcD |
| Description | A relatively novel activation technique, EThcD was used in the LC-MS/MS analysis of tryptic glycopeptides enriched with wheat germ agglutinin from human urine samples. We focused on the characterization of mucin-type O-glycopeptides. EThcD in a single spectrum provided information on both the peptide modified and the glycan carried. Unexpectedly, glycan oxonium ions indicated the presence of O-acetyl, and even O-diacetyl-sialic acids. B and Y fragment ions revealed that i) in core 1 structures the Gal residue featured the O-acetyl-sialic acid, when there was only one in the glycan; ii) several glycopeptides featured core 1 glycans with disialic acids, in certain instances O-acetylated; iii) the disialic acid was linked to the GalNAc residue whatever was the degree of O-acetylation; iv) core 2 isomers with a single O-acetyl-sialic acid were chromatographically resolved. Glycan fragmentation also helped to decipher additional core 2 oligosaccharides: a LacdiNAc-like structure, glycans carrying sialyl LewisX/A at different stages of O-acetylation, and blood antigens. A sialo core 3 structure was also identified. We believe this is the first study when such structures were characterized from a very complex mixture and were linked not only to a specific protein, but also the sites of modifications have been determined. |
| HostingRepository | MassIVE |
| AnnounceDate | 2018-10-25 |
| AnnouncementXML | Submission_2018-10-25_07:32:55.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Eva Hunyadi-Gulyas |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | unknown modification: O-GalNAc glycosylation; unknown modification: O-Acetylation of N-Acetylneuraminic acid |
| Instrument | instrument model: Thermo Scientific Orbitrap Fusion Lumos EASY-ETD |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2018-10-25 06:52:08 | ID requested | |
| ⏵ 1 | 2018-10-25 07:32:56 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Sialic acid O-acetylation, EThCD, Glycopeptides, Lectin Affinity Chromatography |
Contact List
| Zsuzsanna Darula | |
|---|---|
| contact affiliation | Biological Research Centre |
| contact email | darula.zsuzsanna@brc.mta.hu |
| lab head | |
| Eva Hunyadi-Gulyas | |
| contact affiliation | BRC |
| contact email | egulyas@brc.hu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000083070 |
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