PXD011471 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Single amino acid variant . |
Description | We performed deep proteomic profiling down to as few as 9 Panc-1 cells using sample fractionation, TMT multiplexing and carrier/reference strategy. Off line fractionation of the TMT-labeled sample pooled with TMT-labeled carrier Panc-1 whole cell proteome was achieved using alkaline reversed phase spin columns. The fractionation in conjunction with carrier/reference proteome allowed us to detect 47,414 unique peptides derived from 6261 proteins which provided a sufficient coverage to search for single amino acid variants (SAAVs)related to cancer. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-07 |
AnnouncementXML | Submission_2024-10-07_10:20:13.753.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Zhijing Tan |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-10-25 03:38:18 | ID requested | |
⏵ 1 | 2024-10-07 10:20:14 | announced | |
Publication List
10.1021/acs.jproteome.8b00694; |
Tan Z, Yi X, Carruthers NJ, Stemmer PM, Lubman DM, Single Amino Acid Variant Discovery in Small Numbers of Cells. J Proteome Res, 18(1):417-425(2019) [pubmed] |
Keyword List
submitter keyword: Human, Panc-1, LC-MS/MS |
Contact List
David M. Lubman |
contact affiliation | Department of Surgery, University of Michigan, USA. |
contact email | dmlubman@umich.edu |
lab head | |
Zhijing Tan |
contact affiliation | University of Michigan |
contact email | zhijing@umich.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD011471 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011471
- Label: PRIDE project
- Name: Single amino acid variant