PXD011471-1

PXD011471 is an original dataset announced via ProteomeXchange.

Title	Single amino acid variant .
Description	We performed deep proteomic profiling down to as few as 9 Panc-1 cells using sample fractionation, TMT multiplexing and carrier/reference strategy. Off line fractionation of the TMT-labeled sample pooled with TMT-labeled carrier Panc-1 whole cell proteome was achieved using alkaline reversed phase spin columns. The fractionation in conjunction with carrier/reference proteome allowed us to detect 47,414 unique peptides derived from 6261 proteins which provided a sufficient coverage to search for single amino acid variants (SAAVs)related to cancer.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:20:13.753.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhijing Tan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-25 03:38:18	ID requested
⏵ 1	2024-10-07 10:20:14	announced

10.1021/acs.jproteome.8b00694;

Tan Z, Yi X, Carruthers NJ, Stemmer PM, Lubman DM, Single Amino Acid Variant Discovery in Small Numbers of Cells. J Proteome Res, 18(1):417-425(2019) [pubmed]

submitter keyword: Human, Panc-1, LC-MS/MS

David M. Lubman
contact affiliation	Department of Surgery, University of Michigan, USA.
contact email	dmlubman@umich.edu
lab head
Zhijing Tan
contact affiliation	University of Michigan
contact email	zhijing@umich.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD011471

PRIDE project URI

• PRIDE
  1. PXD011471
     1. Label: PRIDE project
     2. Name: Single amino acid variant