PXD011459 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Sequential digestion with Trypsin and Elastase in cross-linking/mass spectrometry |
Description | Cross-linking/mass spectrometry has become an important approach for studying protein structures and protein-protein interactions. The amino acid composition of some protein regions impedes the detection of cross-linked residues, although it would yield invaluable information for protein modelling. Here, we report on a sequential digestion strategy with trypsin and elastase to penetrate regions with a low density of trypsin cleavage sites. We exploited intrinsic substrate recognition proper-ties of elastase to specifically target larger tryptic peptides. Our application of this protocol to the TAF4-12 complex allowed us to identify cross-links in previously inaccessible regions. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:52:39.139.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Therese Dau |
SpeciesList | scientific name: Schizosaccharomyces pombe; NCBI TaxID: 4896; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | carbamoylated residue; monohydroxylated residue |
Instrument | Orbitrap Fusion Lumos; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-10-23 06:10:46 | ID requested | |
1 | 2019-03-07 00:29:35 | announced | |
⏵ 2 | 2024-10-22 04:52:39 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Technical |
submitter keyword: LC-MSMS, cross-linking, yeast,human TAF4-12 complex |
Contact List
Juri Rappsilber |
contact affiliation | Wellcome Centre for Cell Biology, Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Edin-burgh EH9 3BF, U.K. Bioanalytics, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany |
contact email | juri.rappsilber@ed.ac.uk |
lab head | |
Therese Dau |
contact affiliation | University of Edinburgh |
contact email | therese.dau@ed.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD011459 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011459
- Label: PRIDE project
- Name: Sequential digestion with Trypsin and Elastase in cross-linking/mass spectrometry