PXD011458-1

PXD011458 is an original dataset announced via ProteomeXchange.

Title	Mouse and rat liver and liver cell line LC-MS/MS
Description	This study sought to interrogate the effects of lipids and lipid metabolites on the hepatic proteome. Protein expression and tyrosine phosphorylation in high-fat diet (HFD) mouse livers vs. livers of normal chow fed (NC) mice were investigated using multiplexed quantitative LC-MS/MS (TMT labeling). Additionally, the rat hepatoma cell line H4IIE was used to monitor the effects of free fatty acids on tyrosine signaling, gene expression and the levels of reactive oxygen species. These data were used to inform potential points of intervention and chemical perturbations and their effects on phenotypic and signaling output in the cell line, and included small molecule inhibtors of Src-family kinases and antioxidants. Based on the in vitro effects, HFD mice were fed an antioxidant and phenotypic and tyrosine signaling changes were measured.
HostingRepository	PRIDE
AnnounceDate	2019-07-08
AnnouncementXML	Submission_2019-07-08_01:09:14.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011458
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Antje Dittmann
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	Phospho; TMT6plex; Oxidation; MappingA; Carbamidomethyl
Instrument	Q Exactive HF-X; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-23 06:01:46	ID requested
⏵ 1	2019-07-08 01:09:14	announced

Dataset with its publication pending

curator keyword: Biological, Biomedical

submitter keyword: Mouse, rat, liver, phosphotyrosine, LC-MS/MS, diabetes, free fatty acids

Forest Michael White
contact affiliation	Department of Biological Engineering, Koch Institute of Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA
contact email	fwhite@mit.edu
lab head
Antje Dittmann
contact affiliation	MIT
contact email	antje.dittmann@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD011458

PRIDE project URI

• PRIDE
  1. PXD011458
     1. Label: PRIDE project
     2. Name: Mouse and rat liver and liver cell line LC-MS/MS