PXD011452 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative analyses reveal novel roles for N-glycosylation in a major enteric bacterial pathogen |
Description | In eukaryotes glycosylation plays a role in proteome stability, protein quality control and modulating protein function, however, similar studies in bacteria are lacking. Here, we investigate the role of general protein glycosylation systems in bacteria using the enteropathogen Campylobacter jejuni as a well-defined example. By using a quantitative proteomics strategy, we were able to monitor changes in the C. jejuni proteome when glycosylation is disrupted. We demonstrate that in C. jejuni, N-glycosylation is essential to maintain proteome stability and protein quality control. These findings guided us to investigate the role of N-glycosylation in modulating bacterial cellular activities. In glycosylation deficient C. jejuni, the multidrug efflux pump and electron transport pathways were significantly impaired. In vivo, we demonstrate that fully glycosylation deficient C. jejuni were unable to colonise its natural avian host. These results provide the first evidence of a link between proteome stability and complex functions via a bacterial general glycosylation system. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-08 |
AnnouncementXML | Submission_2021-09-08_07:42:48.183.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sherif Abouelhadid |
SpeciesList | scientific name: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819; NCBI TaxID: 192222; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-10-23 01:49:39 | ID requested | |
⏵ 1 | 2021-09-08 07:42:49 | announced | |
Publication List
Abouelhadid S, North SJ, Hitchen P, Vohra P, Chintoan-Uta C, Stevens M, Dell A, Cuccui J, Wren BW, Glycosylation in a Major Enteric Bacterial Pathogen. mBio, 10(2):(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Campylobacter, TMT, quantitative proteomics, Glycosylation |
Contact List
Sherif Abouelhadid |
contact affiliation | Wren Lab London School of Hygiene & Tropical Medicine |
contact email | Sherif.abouelhadid@lshtm.ac.uk |
lab head | |
Sherif Abouelhadid |
contact affiliation | London School of Hygiene & Tropical Medicine |
contact email | sherif.abouelhadid@lshtm.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011452
- Label: PRIDE project
- Name: Quantitative analyses reveal novel roles for N-glycosylation in a major enteric bacterial pathogen