PXD011432-1

PXD011432 is an original dataset announced via ProteomeXchange.

Title	Characterizations of HSP90-interacting complex in renal cells using tandem affinity purification and its potential role in kidney stone formation
Description	Heat shock protein 90 (HSP90) is a highly abundant molecular chaperone that interacts with many other intracellular proteins to regulate various cellular processes. However, compositions of the HSP90-interacting complex remain underinvestigated. This study thus aimed to characterize such complex in human embryonic kidney (HEK293T) cells under normal physiologic state using tandem affinity purification (TAP) followed by protein identification using an ultrahigh-resolution tandem mass spectrometer (Qq-TOF MS/MS). A total of 32 proteins, including four forms of HSP90 and 16 novel HSP90-interacting partners, were successfully identified from this complex using TAP control to subtract non-specific binders. Co-immunoprecipitation followed by immunoblotting and immunofluorescence co-staining confirmed the association of HSP90 with known (HSP70, α-tubulin, and β-actin) and novel (vimentin, calpain-1, and importin-β1) partners. Knockdown of HSP90 by small-interfering RNA (siHSP90) caused significant changes in levels of HSP70, α-tubulin, β-actin, vimentin, and calpain-1, all of which are calcium oxalate (CaOx) crystal-binding proteins that play significant roles in kidney stone formation. Moreover, crystal-binding capability was significantly decreased in siHSP90-transfected cells as compared to non-transfected control and siControl-transfected cells. In summary, we report herein a number of novel HSP90-interacting proteins in renal cells and demonstrate the potential role of HSP90-interacting complex in kidney stone formation.
HostingRepository	PRIDE
AnnounceDate	2018-12-17
AnnouncementXML	Submission_2018-12-17_07:23:06.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011432
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Visith Thongboonkerd
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-22 00:57:34	ID requested
⏵ 1	2018-12-17 07:23:07	announced

Manissorn J, Singhto N, Thongboonkerd V, Characterizations of HSP90-Interacting Complex in Renal Cells Using Tandem Affinity Purification and Its Potential Role in Kidney Stone Formation. Proteomics, 18(24):e1800004(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Chaperone

Heat shock protein 90

HEK293T

Kidney stone

siRNA

TAP tag

Professor Visith Thongboonkerd
contact affiliation	Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, and Center forResearch in Complex Systems Science, Mahidol University, Bangkok, Thailand
contact email	vthongbo@yahoo.com
lab head
Visith Thongboonkerd
contact affiliation	Mahidol University
contact email	sirirajms@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011432
     1. Label: PRIDE project
     2. Name: Characterizations of HSP90-interacting complex in renal cells using tandem affinity purification and its potential role in kidney stone formation