PXD011378-3

PXD011378 is an original dataset announced via ProteomeXchange.

Title	Surface exposure and packing of lipoproteins into Outer Membrane Vesicles are coupled processes in Bacteroides.
Description	Outer membrane vesicles (OMV) are spherical structures derived from the outer membrane (OM) of Gram-negative bacteria. OMVs from the predominant gut microbiota members, Bacteroides, are proposed to play key roles in gut homeostasis. OMV biogenesis in Bacteroides is a poorly understood process. Here, we revisited the protein composition of B. theta OMVs by mass spectrometry. We confirmed that OMVs produced by this organism contain large quantities of glycosidases and proteases, with most of them being lipoproteins. We found that many of these OMV-enriched lipoproteins are encoded by polysaccharide utilization loci (PULs), such as the sus operon. We examined the subcellular localization of the components of the sus operon and found that the alpha-amylase SusG is highly enriched in OMVs while the oligosaccharide importer SusC remains mostly in the OM. We show that all OMV-enriched lipoproteins possess a lipoprotein export sequence (LES) that mediates translocation of SusG from the periplasmic face of the OM towards the extracellular milieu and is required for SusG to localize preferentially to OMVs. We also show that surface-exposed SusG in OMVs is active and can rescue growth of bacterial cells incapable of growing on starch as only carbon source. Our results support the role of OMVs as "public goods" that can be utilized by other organisms with different metabolic capabilities.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:49:41.886.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nichollas Scott
SpeciesList	scientific name: Bacteroides fragilis; NCBI TaxID: 817;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-16 07:49:14	ID requested
1	2018-11-04 13:56:47	announced
2	2018-11-14 03:29:24	announced	Updated publication reference for PubMed record(s): 30404931.
⏵ 3	2024-10-22 04:49:50	announced	2024-10-22: Updated project metadata.

Valguarnera E, Scott NE, Azimzadeh P, Feldman MF, . mSphere, 3(6):(2018) [pubmed]

10.1128/msphere.00559-18;

submitter keyword: OMVs,Bacteroides. LC-MS

Mario Feldman
contact affiliation	Department of Molecular Microbiology, Washington University School of Medicine, 4940 Parkview Place, Campus Box 8230, St. Louis, MO 63110, USA
contact email	mariofeldman@wustl.edu
lab head
Nichollas Scott
contact affiliation	University of Melbourne
contact email	nichollas.scott@unimelb.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011378
     1. Label: PRIDE project
     2. Name: Surface exposure and packing of lipoproteins into Outer Membrane Vesicles are coupled processes in Bacteroides.