PXD011324-2

PXD011324 is an original dataset announced via ProteomeXchange.

Title	GASP and FASP are complementary for LC-MS/MS proteomic analysis of drug-metabolising enzymes and transporters in pig liver
Description	Sample preparation is a critical step in the proteomic workflow. Numerous different approaches are used, tailored to the type of sample, the aims of the experiment, analytical method, and to an extent, user preference. This has resulted in large variation in reported protein abundances. In this study we demonstrate the complementarity of two different sample preparation techniques for the study of absorption, distribution, metabolism and excretion (ADME) related proteins from pig liver tissue. Filter-aided sample preparation (FASP) is a well-established and widely used method, whilst gel-aided sample preparation (GASP) is a relatively new method optimised and simplified from previous gel-associated digestion techniques. To investigate each method the number of peptides and proteins characterised, reproducibility of results and their real-time application were examined. Whilst both methods have their merits and limitations, for example, FASP is the less technical of the two methods, whilst GASP is time efficient, ultimately the two methods show significant differences in the peptides identified and therefore the use of both methods should be considered when examining and quantifying ADME related proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:49:21.495.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martyn Howard
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-10 01:59:54	ID requested
1	2018-11-12 02:32:50	announced
⏵ 2	2024-10-22 04:49:24	announced	2024-10-22: Updated project metadata.

Howard M, Achour B, Al-Majdoub Z, Rostami-Hodjegan A, Barber J, GASP and FASP are Complementary for LC-MS/MS Proteomic Analysis of Drug-Metabolizing Enzymes and Transporters in Pig Liver. Proteomics, 18(24):e1800200(2018) [pubmed]

10.1002/pmic.201800200;

curator keyword: Biological

submitter keyword: GASP, Sample Preparation, FASP, Pig Liver, LC-MS/MS

Jill Barber
contact affiliation	Centre for Applied Pharmacokinetic Research (CAPKR), University of Manchester
contact email	jill.barber@manchester.ac.uk
lab head
Martyn Howard
contact affiliation	University of Manchester, Centre for Applied Pharmacokinetic Research
contact email	martyn.howard@postgrad.manchester.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011324
     1. Label: PRIDE project
     2. Name: GASP and FASP are complementary for LC-MS/MS proteomic analysis of drug-metabolising enzymes and transporters in pig liver