PXD011323-1

PXD011323 is an original dataset announced via ProteomeXchange.

Title	On-chip AP-MS: Interaction proteomics on a microfluidics platform
Description	Here we present a microfluidics-based affinity purification-mass spectrometry (AP-MS) pipeline to identify protein-protein interactions (PPI) using minute amounts of input material. The use of this automated platform allowed us to identify the human cohesin and CCC complex from only 4 micrograms of input lysate, representing a ~50-100 fold downscaling compared to regular microcentrifuge tubes-based workflows. As such, our method holds great promise for future AP-MS applications in which sample amounts are limited.
HostingRepository	PRIDE
AnnounceDate	2022-03-10
AnnouncementXML	Submission_2022-03-10_02:29:13.423.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cristina Furlan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-10 01:53:59	ID requested
⏵ 1	2022-03-10 02:29:13	announced
2	2023-11-14 08:49:47	announced	2023-11-14: Updated project metadata.

10.1038/s41467-019-09533-y;

curator keyword: Technical, Biological

submitter keyword: AP-MS, low input, interaction proteomics

Michiel Vermeulen
contact affiliation	Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Oncode Institute, Radboud University Nijmegen, Nijmegen, The Netherlands
contact email	michiel.vermeulen@science.ru.nl
lab head
Cristina Furlan
contact affiliation	Radboud University
contact email	c.furlan@science.ru.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD011323

PRIDE project URI

• PRIDE
  1. PXD011323
     1. Label: PRIDE project
     2. Name: On-chip AP-MS: Interaction proteomics on a microfluidics platform