PXD011296-1

PXD011296 is an original dataset announced via ProteomeXchange.

Title	High-density chemical cross-linking for modeling protein interactions
Description	We combine several orthogonal cross-linking chemistries as well as improvements in search algorithms, statistical analysis and computational cost to achieve coverage of one unique cross-linked position pair for every 7 amino acids at a 1% false discovery rate. This is accomplished without any peptide-level fractionation, enrichment, or isotopic labeling. We apply our methods to model the complex between a carbonic anhydrase (CA) and its protein inhibitor, as well as the yeast proteasome
HostingRepository	PRIDE
AnnounceDate	2019-12-06
AnnouncementXML	Submission_2019-12-06_07:09:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julian Mintseris
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-05 08:24:53	ID requested
⏵ 1	2019-12-06 07:09:02	announced

Dataset with its publication pending

submitter keyword: Cross-linking

proteasome

Steven P. Gygi
contact affiliation	Department of Cell Biology, Harvard Medical School
contact email	steven_gygi@hms.harvard.edu
lab head
Julian Mintseris
contact affiliation	Harvard Medical School
contact email	julian@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD011296

PRIDE project URI

• PRIDE
  1. PXD011296
     1. Label: PRIDE project
     2. Name: High-density chemical cross-linking for modeling protein interactions