PXD011262 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Temporally & Spatially-Resolved Microproteomics and MALDI MSI Lipidomic Analysis for Traumatic Brain Injury |
Description | Using an experimental TBI rat model of mild/moderate Controlled Cortical Impact (CCI) injury, we combined large-scale proteomics identification and relative quantification using Spatially-Resolved Microproteomics with MALDI MS Imaging of Lipids. Spatially by studying different regions in the brain post injury in a coronal view, with main focus on the injury site itself. Temporally by studying the acute and subacute phase post injury, including injured rat brains at 1 day, 3 days, 7 days, and 10 days post injury. Direct on-tissue micro-digestion followed by micoextraction from 1 mm2 surface area within the injured cortical tissue were subjected to LC-MS & MS/MS analysis using HR MS. In addition, several identified potential biomarkers within our study were used to stimulate dorsal root ganglion (DRG), astrocyte, and macrophage cell lines to obtain a better understanding of their role and contribution in the injury. |
HostingRepository | PRIDE |
AnnounceDate | 2019-06-19 |
AnnouncementXML | Submission_2019-06-19_06:14:11.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jusal Quanico |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-10-03 08:02:13 | ID requested | |
⏵ 1 | 2019-06-19 06:14:12 | announced | |
Publication List
Mallah K, Quanico J, Raffo-Romero A, Cardon T, Aboulouard S, Devos D, Kobeissy F, Zibara K, Salzet M, Fournier I, Mapping Spatiotemporal Microproteomics Landscape in Experimental Model of Traumatic Brain Injury Unveils a link to Parkinson's Disease. Mol Cell Proteomics, 18(8):1669-1682(2019) [pubmed] |
Keyword List
submitter keyword: traumatic brain injury, spatiotemporal microproteomics, long-chain acylcarnitine, macrophage, astrocyte, substantia nigra |
Contact List
Michel Salzet |
contact affiliation | PRISM Laboratory-INSERM U1192, University of Lille, France |
contact email | michel.salzet@univ-lille.fr |
lab head | |
Jusal Quanico |
contact affiliation | PRISM- University of Lille 1 |
contact email | jusal.quanico@univ-lille1.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD011262 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011262
- Label: PRIDE project
- Name: Temporally & Spatially-Resolved Microproteomics and MALDI MSI Lipidomic Analysis for Traumatic Brain Injury