PXD011258 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | MS on EVs from the YSL of 3dpf zebrafish embryos |
Description | We set up a zebrafish model to study EVs in vivo by light microscopy, using CD63-pHluorin as a marker. To verify if exosomes from zebrafish have a comparable composition as human exosomes, and to validate the use of CD63 as a marker, we first analysed the compositon of EVs isolated from in vitro cultured fibroblasts (AB.9 ATCC), and could detect the presence of conventional exosome markers, including (zebrafish) CD63. In live embryos, we could track a population of exosomes from their source (the yolk syncytial layer (YSL)) to their final destination by live microscopy. To assess the composition of these exosomes, we dissociated YSL:CD63-pHluorin expressing 3dpf embryos and isolated exosomes from the supernatant and enriched for EVs from the YSL. This was compared to a background of total EVs isolated the control fish (non-expressing). |
HostingRepository | PRIDE |
AnnounceDate | 2019-04-10 |
AnnouncementXML | Submission_2019-04-10_06:11:42.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Guillaume Arras |
SpeciesList | scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-10-02 09:16:48 | ID requested | |
⏵ 1 | 2019-04-10 06:11:43 | announced | |
Publication List
Verweij FJ, Revenu C, Arras G, Dingli F, Loew D, Pegtel DM, Follain G, Allio G, Goetz JG, Zimmermann P, Herbomel P, Del Bene F, Raposo G, van Niel G, Vivo. Dev Cell, 48(4):573-589.e4(2019) [pubmed] |
Keyword List
submitter keyword: exosomes, zebrafish, endogenous, ex vivo, YSL |
Contact List
Damarys Loew |
contact affiliation | Laboratoire de Spectrometrie de Masse Proteomique Institut Curie PSL Research University |
contact email | Damarys.Loew@curie.fr |
lab head | |
Guillaume Arras |
contact affiliation | Institut Curie |
contact email | guillaume.arras@curie.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD011258 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011258
- Label: PRIDE project
- Name: MS on EVs from the YSL of 3dpf zebrafish embryos