PXD011250-1

PXD011250 is an original dataset announced via ProteomeXchange.

Title	TFIIIC dinamically binds Alu elements to control gene expression through chromatin looping
Description	Folding of the mammalian genome is governed by architectural proteins, such asCTCF. TFIIIC, a RNA polymerase III transcription factor, has been identified as aninsulator but its role in genome topology is totally unknown. Here, we show that TFIIICestablishes long-range genomic interactions that affect gene expression. Upon serumstarvation (SS), TFIIIC occupancy increases at Alu elements (AEs) near promoters ofcell cycle-related genes. Bound AEs become H3K18 hyper-acetylated and fold tocontact distal pre-loaded CTCF sites near other cell cycle genes. The promoters ofthese genes also become hyper-acetylated ensuring their basal transcription during SSand their increased expression during serum re-exposure. Ablation of TFIIIC ordeletion of the TFIIIC-bound AE that loops to the G2/M cycling F (CCNF) locus affectsits expression and nuclear positioning. These results illustrate a novel function ofhuman TFIIIC in changing 3D genome topology through the epigenetic state of AEs.
HostingRepository	PRIDE
AnnounceDate	2020-05-26
AnnouncementXML	Submission_2020-05-26_12:22:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roberto Ferrari
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-02 02:52:37	ID requested
⏵ 1	2020-05-26 12:22:55	announced

Ferrari R, de Llobet Cucalon LI, Di Vona C, Le Dilly F, Vidal E, Lioutas A, Oliete JQ, Jochem L, Cutts E, Dieci G, Vannini A, Teichmann M, de la Luna S, Beato M, TFIIIC Binding to Alu Elements Controls Gene Expression via Chromatin Looping and Histone Acetylation. Mol Cell, 77(3):475-487.e11(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, breast cancer, TFIIIC, Alu

Miguel Beato
contact affiliation	Gene Regulation, Stem Cells and Cancer Program Centre de Regulació Genòmica (CRG) The Barcelona Institute of Science and Technology PRBB Dr. Aiguader 88 E-08003 Barcelona Spain Tlf +34 93 316 0119 Fax +34 93 316 0099 e-mail: miguel.beato@crg.eu Secretary: Gemma Perez Tlf +34 93 316 0165 e-mail: gemma.perez@crg.eu www.crg.eu/4dgenome
contact email	miguel.beato@crg.eu
lab head
Roberto Ferrari
contact affiliation	Center for Genomic Regulation
contact email	roberto.ferrari@crg.eu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD011250

PRIDE project URI

• PRIDE
  1. PXD011250
     1. Label: PRIDE project
     2. Name: TFIIIC dinamically binds Alu elements to control gene expression through chromatin looping