PXD011245-2

PXD011245 is an original dataset announced via ProteomeXchange.

Title	Signaling Pathways in Vasopressin-Mediated Regulation of Aquaporin-2
Description	Vasopressin controls water excretion by regulating the water channel protein, aquaporin 2. Vasopressin acts in the renal collecting duct by binding to a G-protein coupled receptor and activating protein kinase A (PKA). Signaling events beyond PKA are largely unknown. Ultra-deep phosphoproteomic analysis of collecting duct cells isolated from rat kidneys identified and quantified 10,738 phosphorylation sites. Vasopressin significantly altered the abundance of only 219 of these phosphorylation sites, suggesting a highly selective effect on cell signaling. The regulated sites were mapped to cellular processes shown in prior studies to be involved in aquaporin-2 regulation. The mapping and full data set was used to create an online data resource to guide future studies.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:04:19.353.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	CHIN-RANG YANG
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; deaminated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-02 01:59:43	ID requested
1	2020-05-24 22:17:37	announced
⏵ 2	2024-10-22 05:04:20	announced	2024-10-22: Updated project metadata.

10.1152/ajprenal.00281.2019;

Deshpande V, Kao A, Raghuram V, Datta A, Chou CL, Knepper MA, Phosphoproteomic identification of vasopressin V2 receptor-dependent signaling in the renal collecting duct. Am J Physiol Renal Physiol, 317(4):F789-F804(2019) [pubmed]

curator keyword: Biological

submitter keyword: collecting duct,LC-MS/MS, phosphorylation

Mark Knepper
contact affiliation	Epithelial Systems Biology Laboratory, NHLBI, NIH
contact email	knepperm@nhlbi.nih.gov
lab head
CHIN-RANG YANG
contact affiliation	National Institutes of Health, USA
contact email	chin-rang.yang@nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD011245

PRIDE project URI

• PRIDE
  1. PXD011245
     1. Label: PRIDE project
     2. Name: Signaling Pathways in Vasopressin-Mediated Regulation of Aquaporin-2