PXD011232-2

PXD011232 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Comparative phosphoproteomic analysis of barley grains during imbibition
Description	Seed dormancy is an indispensable trait for plants. It of barley is gradually decayed by a period of dry storage (after-ripening) or certain environmental condition. Freshly harvested (FH) and after-ripened (AR) grains have been differentially characterized by numerous studies, such as light, temperature and imbibition. Although FH and AR grains are known to change their gene expression during imbibition, phosphosignaling remain unclear. In this study we aim to investigate the effect of after-ripening on phosphosignaling in our cereal model species, barley. Phosphoproteomic analysis was carried out by using following process. Isolated barley seed embryo was used for protein extraction. Trypsin digestion and phosphopeptide enrichment by HAMMOC method were performed, then purified peptide sample was analyzed by TripleTOF5600 (AB-SCIEX).
HostingRepository	jPOST
AnnounceDate	2019-09-29
AnnouncementXML	Submission_2021-02-04_16:39:52.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shinnosuke ISHIKAWA
SpeciesList	scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationList	S-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-29 02:53:31	ID requested
1	2019-09-28 08:00:07	announced
⏵ 2	2021-02-04 16:39:52	announced	2021-02-04: Updated FTP location.
3	2022-09-18 02:24:10	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: barley, phosphoproteome, seed dormancy, germination, after-ripening

submitter keyword: barley, phosphoproteome, seed dormancy, germination, after-ripening

Contact List

Shinnosuke ISHIKAWA
lab head
Shinnosuke ISHIKAWA
contact affiliation	Tokyo University of Agriculture and Technology
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST000502

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST000502