PXD011211-2

PXD011211 is an original dataset announced via ProteomeXchange.

Title	Chaperonin CCT Checkpoint Function in Basal Transcription Factor TFIID Assembly
Description	TFIID is a cornerstone of eukaryotic gene regulation. Distinct TFIID complexes with unique subunit composition exist and several TFIID subunits are shared with other complexes, conveying intricate cellular decision making to control subunit allocation and functional assembly of this essential transcription factor. However, the underlying molecular mechanisms remain poorly understood. Here, we used quantitative proteomics to examine TFIID submodules and assembly mechanisms in human cells. Structural and mutational analysis of the cytoplasmic TAF5/TAF6/TAF9 submodule identified novel interactions crucial for TFIID integrity, and for allocating TAF9 to TFIID or the SAGA co-activator complex. We discover a key checkpoint function for the chaperonin CCT, which specifically associates with nascent TAF5 for subsequent handover to TAF6/TAF9 and ultimate holo-TFIID formation. Our findings illustrate at the molecular level how multisubunit complexes are crafted in the cell, involving checkpoint decisions facilitated by a chaperone machine.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:49:36.624.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simona Antonova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-27 11:32:02	ID requested
1	2022-03-02 07:12:58	announced
⏵ 2	2023-11-14 08:49:37	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Proteomics , crystal structure, TFIID, chaperonin CCT

Marc Timmers
contact affiliation	Professor of Medical Epigenetics German Cancer Consortium (DKTK), partner site Freiburg German Cancer Research Center (DKFZ) Foundation under Public Law c/o Zentrale Klinische Forschung (ZKF) University Medical Center Freiburg Breisacher Str. 66, 79106 Freiburg, Germany
contact email	m.timmers@dkfz-heidelberg.de
lab head
Simona Antonova
contact affiliation	RMCU, University Medical Center, Utrecht
contact email	s.v.antonova-2@umcutrecht.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD011211

PRIDE project URI

• PRIDE
  1. PXD011211
     1. Label: PRIDE project
     2. Name: Chaperonin CCT Checkpoint Function in Basal Transcription Factor TFIID Assembly