PXD011201-3

PXD011201 is an original dataset announced via ProteomeXchange.

Title	A nuclear RNA export factor variant licenses piRNA-guided heterochromatin formation
Description	Small RNA silencing pathways protect genome integrity in part through establishing heterochromatin at transposon loci. In animals, this process requires piRNA-guided targeting of nuclear PIWI proteins to nascent transcripts. The molecular events contributing to heterochromatin formation upon PIWI binding to nascent RNA, a transient molecule at chromatin, are unknown. Here, we identify SFINX, a protein complex that is required for Piwi-mediated co-transcriptional silencing in Drosophila. It consists of Nxf2—a variant of the nuclear RNA export factor Nxf1/Tap, the mRNA export co-factor Nxt1/p15, and the Piwi-associated protein Panoramix. In the absence of Nxf2, Panoramix is targeted for degradation and piRNA-loaded Piwi is unable to establish heterochromatin. Consequently, nxf2 mutants exhibit severe transposon de-repression and are sterile. We show that within SFINX, Panoramix connects to the heterochromatin machinery while Nxf2 enables target silencing via nascent RNA. Thus, the Nxf2-Nxt1 heterodimer—despite having originated from core mRNA export machinery—has been repurposed for heterochromatin formation. Our data establish an unexpected link between nuclear small RNA biology and NXF-variants, which are widespread in animal lineages, but mostly lack ascribed functions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:48:55.662.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Imre
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	methylthiolated residue; phosphorylated residue; monohydroxylated residue; acetylated residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-27 05:52:55	ID requested
1	2019-06-12 04:57:53	announced
2	2019-08-16 00:25:42	announced	Updated publication reference for PubMed record(s): 31384064.
⏵ 3	2024-10-22 04:49:01	announced	2024-10-22: Updated project metadata.

Batki J, Schnabl J, Wang J, Handler D, Andreev VI, Stieger CE, Novatchkova M, Lampersberger L, Kauneckaite K, Xie W, Mechtler K, Patel DJ, Brennecke J, The nascent RNA binding complex SFiNX licenses piRNA-guided heterochromatin formation. Nat Struct Mol Biol, 26(8):720-731(2019) [pubmed]

10.1038/s41594-019-0270-6;

curator keyword: Biological

submitter keyword: OSC, Silencio,Panoramix, SFINX, Nxf2, Nxt1

Julius Fabian Brennecke
contact affiliation	IMBA - Institute of Molecular Biotechnology Dr. Bohrgasse 3 1030 Vienna Austria
contact email	julius.brennecke@imba.oeaw.ac.at
lab head
Richard Imre
contact affiliation	IMBA - Institute of Molecular Biotechnology
contact email	imre@imp.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011201
     1. Label: PRIDE project
     2. Name: A nuclear RNA export factor variant licenses piRNA-guided heterochromatin formation