PXD011186-1
PXD011186 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The IkB kinase complex is a regulator of mRNA stability |
| Description | U2OS cells were split into two aliquots and cultured in one of the following media (DMEM + 10 % dialysed FCS): i) light medium: contains Arg-0 and Lys-0 ii) heavy medium: contains Arg-10 and Lys-8 Cells were passaged at least three times in the respective medium to ensure complete labelling of proteins. SILAC experiments were performed in a forward and a reverse reaction: for the forward experiment, heavy-labelled cells were irradiated with 20 Gy and harvested 45 minutes after irradiation, while light-labelled cells were left untreated. In the reverse experiment, labels were swapped and light-labelled cells were irradiated, while heavy labelled cells were left untreated. |
| HostingRepository | MassIVE |
| AnnounceDate | 2018-11-23 |
| AnnouncementXML | Submission_2018-11-23_00:20:11.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Daniel Perez Hernandez |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2018-09-25 04:09:09 | ID requested | |
| ⏵ 1 | 2018-11-23 00:20:11 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: mRNA stability, osteosarcoma, proteomics, ikB kinase |
Contact List
| Klaus Scheidereit | |
|---|---|
| contact affiliation | Max Delbrueck Center |
| contact email | scheidereit@mdc-berlin.de |
| lab head | |
| Daniel Perez Hernandez | |
| contact affiliation | Max Delbruck Center |
| contact email | dadapio76@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000082975 |
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