PXD011172-1

PXD011172 is an original dataset announced via ProteomeXchange.

Title	TcI and TcII DTUs isolated from chronic chagasic cardiac patients LC-MSMS
Description	This work aimed to investigate the differential expression of proteins from two distinct genetic groups of T. cruzi (´DTUs´, TcI and TcII), isolated from chronically infected individuals displaying the cardiac form of Chagas’ disease. For this purpose, epimastigote forms of the two isolates were cultured in vitro and the cells recovered for protein extraction. Comparative two-dimensional gel electrophoreses were performed and differentially expressed spots selected for identification by mass spectrometry, followed by database searching and protein categorization. The two-dimensional electrophoretic profiles revealed the complex composition of the T. cruzi extracted proteome. In total 46 differentially expressed proteins were identified present in 40 distinct spots found in the comparative gel analyses. The comparative two-dimensional gel electrophoresis allowed detection of major differences in protein expression between two T. cruzi isolates, belonging to the TcI and TcII genotypes. Our findings suggest that patients displaying the cardiac form of the disease harbor parasites capable of exhibiting distinct proteomic profiles.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-12_14:51:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	William Borges
SpeciesList	scientific name: Trypanosoma cruzi; NCBI TaxID: 5693;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-24 05:41:27	ID requested
⏵ 1	2019-11-12 14:51:16	announced
2	2024-10-22 04:14:24	announced	2024-10-22: Updated project metadata.

Tavares de Oliveira M, Taciana Santos Silva K, Xavier Neves L, de Ornelas Toledo MJ, Castro-Borges W, de Lana M, Differential expression of proteins in genetically distinct Trypanosoma cruzi samples (TcI and TcII DTUs) isolated from chronic Chagas disease cardiac patients. Parasit Vectors, 11(1):611(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: T. cruzi

DTUs

Differential Proteomics

Chagas's disease

William Castro Borges
contact affiliation	Departamento de Ciências Biológicas, Laboratório de Enzimologia e Proteômica, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Brasil
contact email	wborges@ufop.edu.br
lab head
William Borges
contact affiliation	Universidade Federal de Ouro Preto
contact email	wborges@ufop.edu.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD011172

PRIDE project URI

• PRIDE
  1. PXD011172
     1. Label: PRIDE project
     2. Name: TcI and TcII DTUs isolated from chronic chagasic cardiac patients LC-MSMS