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PXD011172 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTcI and TcII DTUs isolated from chronic chagasic cardiac patients LC-MSMS
DescriptionThis work aimed to investigate the differential expression of proteins from two distinct genetic groups of T. cruzi (´DTUs´, TcI and TcII), isolated from chronically infected individuals displaying the cardiac form of Chagas’ disease. For this purpose, epimastigote forms of the two isolates were cultured in vitro and the cells recovered for protein extraction. Comparative two-dimensional gel electrophoreses were performed and differentially expressed spots selected for identification by mass spectrometry, followed by database searching and protein categorization. The two-dimensional electrophoretic profiles revealed the complex composition of the T. cruzi extracted proteome. In total 46 differentially expressed proteins were identified present in 40 distinct spots found in the comparative gel analyses. The comparative two-dimensional gel electrophoresis allowed detection of major differences in protein expression between two T. cruzi isolates, belonging to the TcI and TcII genotypes. Our findings suggest that patients displaying the cardiac form of the disease harbor parasites capable of exhibiting distinct proteomic profiles.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWilliam Borges
SpeciesList scientific name: Trypanosoma cruzi; NCBI TaxID: 5693;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-09-24 05:41:27ID requested
12019-11-12 14:51:16announced
Publication List
Tavares de Oliveira M, Taciana Santos Silva K, Xavier Neves L, de Ornelas Toledo MJ, Castro-Borges W, de Lana M, Differential expression of proteins in genetically distinct Trypanosoma cruzi samples (TcI and TcII DTUs) isolated from chronic Chagas disease cardiac patients. Parasit Vectors, 11(1):611(2018) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: T. cruzi
Differential Proteomics
Chagas's disease
Contact List
William Castro Borges
contact affiliationDepartamento de Ciências Biológicas, Laboratório de Enzimologia e Proteômica, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Brasil
contact emailwborges@ufop.edu.br
lab head
William Borges
contact affiliationUniversidade Federal de Ouro Preto
contact emailwborges@ufop.edu.br
dataset submitter
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Dataset FTP location
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