PXD011105-3

PXD011105 is an original dataset announced via ProteomeXchange.

Title	Proteomic characterization of a hyper-virulent isolate of Pseudomonas aeruginosa
Description	Taxonomic outliers of Pseudomonas aeruginosa recently emerged as infectious for humans. Here we present the first analysis of a hyper-virulent isolate that cause hemorrhagic pneumonia. We demonstrated that, in two sequential clones CLJ1 and CLJ3 recovered from a patient with chronic obstructive pulmonary disease undergoing antibiotic therapy, insertion of a mobile genetic element into the P. aeruginosa chromosome affected major virulence-associated phenotypes and led to increased resistance to antibiotics used to treat the patient. Our work reveals insertion sequences as major players in enhancing the pathogenic potential of a P. aeruginosa taxonomic outlier by modulating both the virulence and resistance to antimicrobials. This also explains the ability of this bacterium to adapt to an infected host and cause a serious disease.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:48:48.845.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Pseudomonas aeruginosa; NCBI TaxID: 287;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-17 01:27:01	ID requested
1	2019-03-18 10:05:43	announced
2	2019-10-25 03:54:37	announced	2019-10-25: Updated publication reference for PubMed record(s): 30946644.
⏵ 3	2024-10-22 04:48:57	announced	2024-10-22: Updated project metadata.

Sentausa E, Basso P, Berry A, Adrait A, Bellement G, Cout, é Y, Lory S, Elsen S, Attr, é, e I, Insertion sequences drive the emergence of a highly adapted human pathogen. Microb Genom, 6(9):(2020) [pubmed]

10.1099/mgen.0.000265;

curator keyword: Biomedical

submitter keyword: virulence, adaptation,Pseudomonas aeruginosa, antibiotic resistance, nanoLC-MS/MS

Virginie Brun
contact affiliation	EDyP
contact email	virginie.brun@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD011105

PRIDE project URI

• PRIDE
  1. PXD011105
     1. Label: PRIDE project
     2. Name: Proteomic characterization of a hyper-virulent isolate of Pseudomonas aeruginosa