PXD011103-1

PXD011103 is an original dataset announced via ProteomeXchange.

Title	A non-canonical SWI/SNF complex underlies synthetic lethality in cancers driven by BAF complex perturbation
Description	Mammalian SWI/SNF chromatin remodeling complexes exist in three distinct, final-form assemblies: canonical BAF (cBAF), PBAF, and a newly characterized non-canonical complex, ncBAF. However, their complex-specific targeting on chromatin, functions and roles in disease remain largely unknown. Here, we comprehensively map complex assemblies on chromatin and find that ncBAF uniquely localizes to CTCF sites and promoters. We identified ncBAF subunits as major synthetic lethalities specific to human synovial sarcoma and malignant rhabdoid tumor, which share in common cBAF complex perturbation. Chemical and biological depletion of the BRD9 subunit of ncBAF rapidly attenuates SS and MRT cell proliferation. Notably, in cBAF-perturbed cancers, ncBAF complexes retain localization to CTCF sites and promoters, and maintain gene expression at retained mSWI/SNF sites to support cell proliferation in a manner distinct from fusion oncoprotein-mediated targeting. Taken together, these findings unmask the unique targeting and function of ncBAF complexes and present new cancer-specific therapeutic targets.
HostingRepository	PRIDE
AnnounceDate	2018-11-06
AnnouncementXML	Submission_2018-11-06_08:43:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew D'Avino
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-17 01:22:30	ID requested
⏵ 1	2018-11-06 08:43:59	announced
2	2024-10-22 04:49:54	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: mSWI/SNF, BAF, chromatin remodeling, BRD7, BRD9, DPF2

Cigall Kadoch
contact affiliation	Dana Farber Cancer Institute
contact email	Cigall_Kadoch@dfci.harvard.edu
lab head
Andrew D'Avino
contact affiliation	Broad Institute of MIT and Harvard
contact email	davino@broadinstitute.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD011103

PRIDE project URI

• PRIDE
  1. PXD011103
     1. Label: PRIDE project
     2. Name: A non-canonical SWI/SNF complex underlies synthetic lethality in cancers driven by BAF complex perturbation