PXD011092 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Crosslinking Mapping of yeast Komagataella phaffii TFIID complex |
Description | Transcription preinitiation complex assembly on the promoters of protein encoding genes is nucleated in vivo by TFIID composed of the TATA-box Binding Protein (TBP) and 13 TBP-associate factors (Tafs) providing regulatory and chromatin binding functions. We used CXMS to study the organization and structure of the purified TFIID complex from Komagataella phaffii. Together with Cryo-EM, We confirm the unique subunit stoichiometry prevailing in TFIID and uncover a hexameric arrangement of Tafs containing a HF domain. Interaction with promoter DNA highlights two non-selective binding sites consistent with a DNA scanning mode. |
HostingRepository | PRIDE |
AnnounceDate | 2018-10-08 |
AnnouncementXML | Submission_2018-10-08_05:41:43.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jie Luo |
SpeciesList | scientific name: Komagataella phaffii; NCBI TaxID: 460519; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-09-14 01:45:07 | ID requested | |
⏵ 1 | 2018-10-08 05:41:44 | announced | |
2 | 2019-05-14 07:21:46 | announced | Updated publication reference for PubMed record(s): 30975984. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: K. phaffii TFIID CXMS BS3 |
Contact List
Jeff Ranish |
contact affiliation | Institute for Systems Biology, Seattle, WA 98109 |
contact email | jranish@systemsbiology.org |
lab head | |
Jie Luo |
contact affiliation | Institute for Systems Biology |
contact email | jluo@systemsbiology.org |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD011092 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011092
- Label: PRIDE project
- Name: Crosslinking Mapping of yeast Komagataella phaffii TFIID complex