PXD011087-1

PXD011087 is an original dataset announced via ProteomeXchange.

Title	Shotgun proteomic profiling of intestinal mucosa of rainbow trout Oncorhynchus mykiss
Description	The objective of this study was to identify and quantify proteomic profiles of intestine of rainbow trout (Oncorhynchus mykiss). Specific pathogen free rainbow trout (mean length 15 ± 1 cm) were maintained in recirculating de-chlorinated water at 19±1 °C. Prior to the experiment, fish were distributed between aquaria. The test groups were infected by immersion of Yersinia ruckeri CSF007-82 (biotype 1) and 7959-11 (biotype 2) strains. The control group was immersed similar with sterile broth medium. Fish were anaesthetized and sampled aseptically at different time points. Each intestine was washed three times with sterile phosphate-buffered saline containing a cocktail of mammalian protease inhibitors. Intestinal mucosa was scraped with a sterile large scalpel blade. Intestinal samples were snap-frozen in liquid nitrogen and stored at –80 °C.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-12_13:59:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gokhlesh Kumar
SpeciesList	scientific name: Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri); NCBI TaxID: 8022;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-14 00:41:22	ID requested
⏵ 1	2019-11-12 13:59:24	announced

Kumar G, Hummel K, Razzazi-Fazeli E, El-Matbouli M, Modulation of posterior intestinal mucosal proteome in rainbow trout (Oncorhynchus mykiss) after Yersinia ruckeri infection. Vet Res, 50(1):54(2019) [pubmed]

curator keyword: Biological

submitter keyword: Rainbow trout, intestine, LC MS-MS, Proteome profile

Dr. Gokhlesh Kumar
contact affiliation	Clinical Division of Fish Medicine, University of Veterinary Medicine, Vienna, Austria
contact email	gokhlesh.kumar@vetmeduni.ac.at
lab head
Gokhlesh Kumar
contact affiliation	University of Veterinary Medicine, Vienna
contact email	Gokhlesh.Kumar@vetmeduni.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD011087

PRIDE project URI

• PRIDE
  1. PXD011087
     1. Label: PRIDE project
     2. Name: Shotgun proteomic profiling of intestinal mucosa of rainbow trout Oncorhynchus mykiss