PXD011085-2

PXD011085 is an original dataset announced via ProteomeXchange.

Title	Anticancer effect of deuterium depleted water - comprehensive proteomics analysis suggests a redox disbalance mechanism
Description	The proteomics data is derived from 4 parts of samples prepared and analysed by nanoLC-MS/MS. In order to make appropriate statistical analysed could be performed, all treatments were performed in biological replicates. The number of replicates was 4 for protein abundances analysis (part 1), 2 for time course analysis (part 2), 3 for redox proteomics analysis (part 3) and 2 for TPP analysis (part 4). Each set of TMT10 labelling samples were combined and fractionationed into 10 fractions. In total, 113 TMT labelling samples including 11 sets of TMT10 and 3 sets of iodoTMT6 were performed with a 120 min gradient. In each part of the experiment, separate controls treated with the vehicle were included. Samples were analysed in random order to reduce the “order of injection” effect. In part 1, A549 cells were treated with CAMP, MTX, PCTL and 80 ppm DDW (20 samples). In part 2, the cells were treated 80 ppm DDW, MTX and PCTL for 4-48h (5 time points, 40 samples). In addition, 2 control samples treated with the vehicle for 48h were included in each set of TMT10 for normalization. In part 3, the cells were treated with 80 ppm DDW and auranofin (3 samples). Finally, in part 4, the cells were treated with 80 ppm DDW and afterwords the cells were treated with 10 temperatures (40 samples). Quality check was performed by calculating the variation (CV) between the replicates as well as by building PCA models to verify the small overall spread between the replicates.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:48:52.431.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xuepei Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-13 08:45:25	ID requested
1	2019-09-26 05:44:06	announced
⏵ 2	2024-10-22 04:49:01	announced	2024-10-22: Updated project metadata.

10.1074/mcp.ra119.001455;

ProteomeXchange project tag: Human Proteome Project

submitter keyword: anticancer, proteomics, redox disbalance,deuterium depleted water

Roman A Zubarev
contact affiliation	Karolinska Institute
contact email	Roman.Zubarev@ki.se
lab head
Xuepei Zhang
contact affiliation	Karolinska Institutet
contact email	xuepei.zhang@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD011085

PRIDE project URI

• PRIDE
  1. PXD011085
     1. Label: PRIDE project
     2. Name: Anticancer effect of deuterium depleted water - comprehensive proteomics analysis suggests a redox disbalance mechanism